耦合 HSQC 实验在整料结构解析中的应用——以三叶苦木中的三种复杂皂甙为例。
Utility of coupled-HSQC experiments in the intact structural elucidation of three complex saponins from Blighia sapida.
机构信息
University of Maryland-FDA Joint Institute, College Park, MD 20742, USA.
出版信息
Carbohydr Res. 2011 May 1;346(6):759-68. doi: 10.1016/j.carres.2011.02.019. Epub 2011 Feb 25.
Abstract
The structures of three complex saponins from the fruit pods of Blighia sapida have been elucidated and their (1)H and (13)C NMR spectra assigned employing a variety of one- and two-dimensional NMR techniques without degradative chemistry. The saponins have either four or six monosaccharide units linked to a triterpene aglycone. High-resolution, proton-coupled-HSQC spectra were important for determining both the identities of the intact monosaccharide units and coupling constants in strongly coupled proton spin systems. These NMR experiments will prove crucial as the complexity of saponin structures reaches the limit that can be determined solely by NMR.
摘要
三种复杂的荜茇果皂素的结构已被阐明，并采用各种一维和二维 NMR 技术对其（1）H 和（13）C NMR 谱进行了归属，而无需进行降解化学处理。这些皂素具有四个或六个单糖单元与三萜苷元相连。高分辨率质子偶合 HSQC 谱对于确定完整单糖单元的身份和强偶合质子自旋系统中的耦合常数都非常重要。这些 NMR 实验将非常重要，因为随着皂素结构的复杂性达到仅通过 NMR 确定的极限，这些实验将变得至关重要。
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