Shimamoto K, Nakagawa M, Iimura O
Second Department of Internal Medicine, Sapporo Medical College, Japan.
Horm Metab Res Suppl. 1990;22:75-9.
In the investigation of hypotensive mechanisms for the converting enzyme (ACE) inhibitor, precise methods for plasma kinin and angiotensin II measurement are essential. We describe here determination methods of plasma kinin and angiotensin II, and their applications to converting enzyme inhibitor administration. In our kinin RIA system, a high titered antiserum (final dilution 1:640,000) was used. Sensitivity was 0.25 pg/tube. Regarding plasma kinin measurement, an inhibitor mixture including aprotinin, hexadimethrine, SBTI, phenanthroline and EDTA, was used to collect the plasma sample. The determined plasma kinin levels in normal subjects were below 10 pg/ml. A very sensitive and simplified direct RIA system for plasma angiotensin II was also developed in our laboratory using the very high titered antiserum (final dilution 1:1,500,000). The sensitivity was 0.1 pg/tube, and cross-reactivity of angiotensin I was less than 0.1%. These data suggest that plasma angiotensin II level can be determined directly in very small plasma samples, such as 0.1 ml by this RIA system even in the ACE inhibitor administration. The plasma angiotensin II levels in normal subjects were 12.0 pg/ml. Both of these RIAs which were applied to the clinical experiments of ACE inhibitor administration.
在研究转化酶（ACE）抑制剂的降压机制时，精确测量血浆激肽和血管紧张素II的方法至关重要。我们在此描述血浆激肽和血管紧张素II的测定方法及其在转化酶抑制剂给药中的应用。在我们的激肽放射免疫分析系统中，使用了高滴度抗血清（最终稀释度为1:640,000）。灵敏度为0.25 pg/管。关于血浆激肽测量，使用了一种包含抑肽酶、己二烯二胺、大豆胰蛋白酶抑制剂、邻菲罗啉和乙二胺四乙酸的抑制剂混合物来采集血浆样本。正常受试者测定的血浆激肽水平低于10 pg/ml。我们实验室还使用高滴度抗血清（最终稀释度为1:1,500,000）开发了一种非常灵敏且简化的血浆血管紧张素II直接放射免疫分析系统。灵敏度为0.1 pg/管，血管紧张素I的交叉反应性小于0.1%。这些数据表明，即使在给予ACE抑制剂的情况下，通过该放射免疫分析系统也可以在非常少量的血浆样本（如0.1 ml）中直接测定血浆血管紧张素II水平。正常受试者的血浆血管紧张素II水平为12.0 pg/ml。这两种放射免疫分析方法均应用于ACE抑制剂给药的临床实验。
