单纯疱疹病毒的等电聚焦
Isoelectric focusing of herpes simplex virus.
作者信息
Olofsson S
出版信息
Arch Virol. 1975;49(2-3):93-8. doi: 10.1007/BF01317529.
PMID:2144
Abstract
Herpes simplex virus was inserted into a preformed stable pH gradient and electrofocused for about one hour. Dextran was used as the density gradient forming agent. Virions banded at pH 4.9+/-0.1 and nucleocapsids at 4.1+/-0.05. About 10-20 per cent of infective virus was recovered.
摘要
将单纯疱疹病毒插入预先形成的稳定pH梯度中，并进行电聚焦约一小时。使用葡聚糖作为密度梯度形成剂。病毒粒子在pH 4.9±0.1处形成条带，核衣壳在pH 4.1±0.05处形成条带。回收了约10%-20%的感染性病毒。
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