Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Gent, Belgium.
Water Res. 2011 Apr;45(9):2845-54. doi: 10.1016/j.watres.2011.03.005. Epub 2011 Mar 10.
Effluents of anaerobic wastewater treatment plants are saturated with methane, an effective greenhouse gas. We propose a novel approach to treat such effluents using a coculture of methane oxidizing communities and microalgae, further indicated as methalgae, which would allow microbial methane oxidation with minimal CO(2) emissions. Coculturing a methane oxidizing community with microalgae in sequence batch reactors under continuous lightning yielded a factor of about 1.6 more biomass relative to the control without microalgae. Moreover, 55% less external oxygen supply was needed to maintain the methane oxidation, as oxygen was produced in situ by the microalgae. An overall methane oxidation rate of 171±27 mg CH(4) L(-1) liquid phase d(-1) was accomplished in a semi-batch setup, while the excess CO(2) production was lower than 1mg CO(2) L(-1) d(-1). Both nitrate and ammonium were feasible nitrogen sources for the methalgae. These results show that a coculture of microalgae and methane oxidizing communities can be used to oxidize dissolved methane under O(2)-limiting conditions, which could lead to a novel treatment for dissolved methane in anaerobic effluents.
厌氧废水处理厂的流出物中充满了甲烷，这是一种有效的温室气体。我们提出了一种使用甲烷氧化群落和微藻（进一步表示为 methalgae）的共培养物来处理这种流出物的新方法，这将允许微生物进行甲烷氧化，同时最大限度地减少 CO(2) 排放。在连续光照下，将甲烷氧化群落与微藻序列分批培养在序列分批反应器中，与没有微藻的对照相比，生物量增加了约 1.6 倍。此外，由于微藻在原地产生氧气，因此需要较少的外部氧气供应来维持甲烷氧化，减少了 55%。在半分批设置中，实现了 171±27 mg CH(4) L(-1) 液相 d(-1) 的整体甲烷氧化速率，而过量的 CO(2) 产生量低于 1mg CO(2) L(-1) d(-1)。硝酸盐和铵盐都是 methalgae 的可行氮源。这些结果表明，微藻和甲烷氧化群落的共培养物可用于在 O(2)限制条件下氧化溶解甲烷，这可能为厌氧流出物中的溶解甲烷提供一种新的处理方法。
