Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
DSMZ - Deutsche Sammlung für Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):397-402. doi: 10.1099/ijs.0.031138-0. Epub 2011 Mar 25.
A Gram-stain-positive, aerobic, non-motile, psychrophilic bacterium, designated strain Cr6-08(T), was isolated from alpine glacier cryoconite. Growth of strain Cr6-08(T) occurred at 1-25 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Cr6-08(T) is most closely related to members of the genus Arthrobacter. Strain Cr6-08(T) possessed chemotaxonomic properties consistent with those of the genus Arthrobacter, such as peptidoglycan type A3α (l-Lys-L-Ala(4)), MK-9(H(2)) as major menaquinone and anteiso- and iso-branched compounds (anteiso-C(15 : 0) and iso-C(15 : 0)) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown glycolipid and three unknown polar lipids. The genomic DNA G+C content of strain Cr6-08(T) was 57.3 mol%. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain Cr6-08(T) is considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter cryoconiti sp. nov. is proposed. The type strain is Cr6-08(T) ( = DSM 23324(T) = LMG 26052(T) = CGMCC 1.10698(T)).
一株革兰氏阳性、需氧、不运动、嗜冷的细菌,被命名为 Cr6-08(T) 菌株,从高山冰川冰核中分离得到。Cr6-08(T) 菌株的生长温度为 1-25°C。基于 16S rRNA 基因序列的系统发育分析表明,Cr6-08(T) 菌株与节杆菌属的成员最为密切相关。Cr6-08(T) 菌株具有与节杆菌属一致的化学分类特征,例如肽聚糖类型 A3α(l-Lys-L-Ala(4))、MK-9(H(2)) 作为主要的类异戊二烯醌和直链和支链化合物(anteiso-C(15 : 0) 和 iso-C(15 : 0))作为主要的细胞脂肪酸。极性脂谱包含二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、一种未知的糖脂和三种未知的极性脂。Cr6-08(T) 菌株的基因组 DNA G+C 含量为 57.3 mol%。基于表型和化学分类特征、系统发育分析和 DNA-DNA 同源性数据,Cr6-08(T) 菌株被认为代表了节杆菌属的一个新种,因此提议将其命名为 Arthrobacter cryoconiti sp. nov.。该模式菌株为 Cr6-08(T)(=DSM 23324(T)=LMG 26052(T)=CGMCC 1.10698(T))。