Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa.
Molecules. 2011 Mar 24;16(4):2754-65. doi: 10.3390/molecules16042754.
The methanolic and aqueous extracts of Garcinia kola seeds were screened for their anti-Vibrio activities against 50 Vibrio isolates obtained from wastewater final effluents in the Eastern Cape Province, South Africa. The crude extracts at 10 mg/mL exhibited appreciable inhibitory activities against most of the test Vibrio isolates, with zones of inhibition ranging from 10-19 mm for methanol extract and 8-15 mm for the aqueous extracts. The minimum inhibitory concentrations (MIC) of the methanol extract varied from 0.313 to 2.5 mg/mL while that for the aqueous extract was 10 mg/mL for all the susceptible Vibrio isolates. Rate of kill assay of the methanolic extracts against three selected Vibrio species showed bacteriostatic activities against all of them achieving 58% and 60% (Vibrio vulnificus AL042); 68% and 69% (Vibrio parahaemolyticus AL049); and 70% and 78% (Vibrio fluvialis AL040) killing of the test bacteria at 3× and 4 ×MIC values, respectively, after 2 h exposure time. We conclude that Garcinia kola seeds hold promise as a potential source of therapeutic compounds of relevance in Vibrio infections management.
考来拉果的甲醇和水提取物被筛选出具有抗弧菌活性，针对的是从南非东开普省废水最终排放中获得的 50 个弧菌分离株。粗提取物在 10mg/mL 时对大多数测试弧菌分离株表现出明显的抑制活性，甲醇提取物的抑制带范围为 10-19mm，水提取物的抑制带范围为 8-15mm。甲醇提取物的最小抑菌浓度（MIC）从 0.313 到 2.5mg/mL 不等，而水提取物对所有敏感弧菌分离株的 MIC 均为 10mg/mL。对三种选定的弧菌种的甲醇提取物的杀菌率测定表明，所有三种提取物均具有抑菌活性，在 2 小时暴露时间后，在 3×和 4×MIC 值时，对测试细菌的杀灭率分别达到 58%和 60%（创伤弧菌 AL042）；68%和 69%（副溶血性弧菌 AL049）；以及 70%和 78%（弗氏柠檬酸杆菌 AL040）。我们得出结论，考来拉果种子有望成为治疗与弧菌感染管理相关的化合物的潜在来源。
