Laboratory of Plant Virology, Department of Cellular Biology, Biological Sciences Institute, University of Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, Brasília, Distrito Federal, 70910-900, Brazil.
Arch Virol. 2011 Aug;156(8):1425-9. doi: 10.1007/s00705-011-0973-4. Epub 2011 Mar 26.
Groundnut ringspot virus is a negative-sense single-stranded RNA virus that belongs to the genus Tospovirus and is the prevalent member of this genus in Brazil. This work presents the nucleotide sequence of the L RNA, with a single open reading frame of 2873 amino acids in the complementary strand corresponding to the RNA-dependent RNA polymerase (L protein), as well as the characterization of conserved domains of the L protein by in silico analysis. Phylogenetic analysis of different L protein domains confirmed that GRSV is a member of the American clade, and comparison with a N-protein indicates that phylogeny based on L protein sequences may be more reliable than that based on the N protein.
落花生环斑病毒是一种负义单链 RNA 病毒，属于布尼亚病毒科，是巴西流行的该属成员。本工作介绍了 L 核糖核酸的核苷酸序列，互补链上有一个单一的开放阅读框，编码 2873 个氨基酸，对应于 RNA 依赖性 RNA 聚合酶（L 蛋白），以及通过计算机分析对 L 蛋白保守结构域的特征描述。不同 L 蛋白结构域的系统发育分析证实，GRSV 是美洲分支的成员，与 N 蛋白的比较表明，基于 L 蛋白序列的系统发育可能比基于 N 蛋白的系统发育更可靠。
