Trofimiuk Emil, Braszko Jan J
Department of Clinical Pharmacology, Medical University of Bialystok, Waszyngtona 15A, 15-274, Bialystok, Poland.
Lipids. 2011 May;46(5):417-23. doi: 10.1007/s11745-011-3551-3. Epub 2011 Mar 26.
Cod liver oil (CLO) is a rich source of omega-3 fatty acids (FA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The existing data suggest that EPA and DHA are the active agents of fish oil. In this study, we tested a hypothesis that the active constituents of CLO alleviate the negative impact of prolonged restraint stress on cognitive functions of male Wistar rats. Specifically, we attempted to characterize the preventive action of long-lasting treatment with CLO [0.375 ml/100 g body weight (equivalent to a dose of 300 mg/kg DHA and 225 mg/kg EPA), p.o. for 21 days] against an impairment caused by chronic restraint stress (2 h daily for 21 days) on recall as tested in a passive avoidance situation and on the spatial reference and working memory tested in a Barnes maze as well as on locomotor activity and anxiety behavior tested respectively in an open field and elevated plus-maze. We found that CLO administration statistically significantly (p < 0.01, both) prevented the deleterious effects of chronic restraint stress on recall and the spatial memory.
鱼肝油（CLO）是ω-3脂肪酸（FA）的丰富来源，尤其是二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）。现有数据表明，EPA和DHA是鱼油的活性成分。在本研究中，我们检验了一个假设，即CLO的活性成分可减轻长期束缚应激对雄性Wistar大鼠认知功能的负面影响。具体而言，我们试图描述用CLO [0.375 ml/100 g体重（相当于300 mg/kg DHA和225 mg/kg EPA的剂量），口服，持续21天]进行长期治疗对慢性束缚应激（每天2小时，持续21天）所导致的记忆损伤的预防作用，该记忆损伤通过被动回避实验检测回忆能力，通过巴恩斯迷宫检测空间参考记忆和工作记忆，以及分别通过旷场实验和高架十字迷宫检测运动活动和焦虑行为。我们发现，给予CLO在统计学上具有显著意义（两者均p < 0.01），可预防慢性束缚应激对回忆和空间记忆的有害影响。
