Department of Pathology, Kinki University School of Medicine, 377-2 Ohno-higashi, Osakasayama, Osaka, 589-8511, Japan.
J Neural Transm (Vienna). 2011 Sep;118(9):1263-72. doi: 10.1007/s00702-011-0629-2. Epub 2011 Mar 27.
Exercise is reported to inhibit neuronal apoptotic cell death in the hippocampus and improve learning and memory. However, the effect of exercise on inhibition of neuronal apoptosis surrounding the area of damage after traumatic brain injury (TBI) and the improvement of cerebral dysfunction following TBI are unknown. Here, we investigate the effect of exercise on morphology and cerebral function following TBI in rats. Wistar rats received TBI by a pneumatic controlled injury device were randomly divided into two groups: (1) non-exercise group and (2) exercise group. The exercise group ran on a treadmill for 30 min/day at 22 m/min for seven consecutive days. Immunohistochemical and behavioral studies were performed following TBI. The number of single-stranded DNA (ssDNA)-positive cells around the damaged area early after TBI was significantly reduced in the exercise group compared with the non-exercise group (P < 0.05). Furthermore, most ssDNA-positive cells in the non-exercise group co-localized with neuronal cells. However, in the exercise group, a few ssDNA-positive cells co-localized with neurons. In addition, there was a significant increase in neuronal cell number and improvement in cerebral dysfunction after TBI in the exercise group compared with the non-exercise group (P < 0.05). These results indicate that exercise following TBI inhibits neuronal degeneration and apoptotic cell death around the damaged area, which results in improvement of cerebral dysfunction. In summary, treadmill running improved cerebral dysfunction following TBI, indicating its potential as an effective clinical therapy. Therefore, exercise therapy (rehabilitation) in the early phase following TBI is important for recuperation from cerebral dysfunction.
运动被报道可抑制海马区神经元凋亡细胞死亡，并改善学习和记忆。然而，运动对创伤性脑损伤（TBI）后损伤区域周围神经元凋亡的抑制作用以及对 TBI 后大脑功能障碍的改善作用尚不清楚。在这里，我们研究了运动对 TBI 大鼠形态和大脑功能的影响。使用气动控制损伤装置对 Wistar 大鼠进行 TBI，然后将其随机分为两组：（1）非运动组和（2）运动组。运动组在连续 7 天内每天以 22m/min 的速度在跑步机上跑步 30 分钟。TBI 后进行免疫组织化学和行为学研究。与非运动组相比，运动组 TBI 后早期损伤区域周围的单链 DNA（ssDNA）阳性细胞数量明显减少（P<0.05）。此外，非运动组中的大多数 ssDNA 阳性细胞与神经元细胞共定位。然而，在运动组中，少数 ssDNA 阳性细胞与神经元共定位。此外，与非运动组相比，运动组神经元细胞数量增加，TBI 后大脑功能障碍得到改善（P<0.05）。这些结果表明，TBI 后运动可抑制损伤区域周围的神经元变性和凋亡细胞死亡，从而改善大脑功能障碍。总之，跑步机运动改善了 TBI 后的大脑功能障碍，表明其具有作为有效临床治疗的潜力。因此，TBI 后早期进行运动疗法（康复）对于大脑功能障碍的恢复很重要。
