Interfakultaeres Institut fuer Zellbiologie, Abteilung Immunologie, Auf der Morgenstelle 15, 72076, Tuebingen, Germany.
Cell Stress Chaperones. 2011 Sep;16(5):495-504. doi: 10.1007/s12192-011-0261-y. Epub 2011 Mar 26.
Due to their adjuvant effect and their ability to chaperone tumor-associated peptides, heat shock proteins constitute a potent alarm signal for the immune system and can lead to activation of anti-tumor T-cell immunity. Radiofrequency ablation has been reported to induce heat shock protein expression especially that of heat shock protein 70 in sublethally damaged tumor cells. In this study, we evaluated the release of heat shock protein 70 into the serum of cancer-bearing patients directly after radiofrequency ablation. Sera of 22 patients undergoing radiofrequency ablation for the treatment of primary and secondary malignancies of the liver, kidney, and lung, as well as control sera of 20 patients undergoing diagnostic liver biopsy were analyzed using a manufactured heat shock protein 70 ELISA. A significant increase in serum levels of heat shock protein 70 was detectable in the patient cohort 1 day after radiofrequency ablation. More than a twofold increase was observed in nine out of 22 patients, which tended to correlate with favorable clinical outcome. No patient of the control group revealed a comparable increase. Radiofrequency ablation can lead to a release of heat shock protein 70 into the serum, which is transiently detectable 1 day after treatment. Elevated heat shock protein 70 serum levels may constitute a biomarker for favorable clinical outcome.
由于其佐剂作用和伴侣肿瘤相关肽的能力，热休克蛋白构成了免疫系统的有效警报信号，并能导致抗肿瘤 T 细胞免疫的激活。射频消融术已被报道可诱导热休克蛋白表达，特别是亚致死性损伤的肿瘤细胞中的热休克蛋白 70。在这项研究中，我们评估了射频消融后直接在荷瘤患者血清中热休克蛋白 70 的释放。使用制造的热休克蛋白 70 ELISA 分析了 22 例因原发性和继发性肝、肾、肺恶性肿瘤而行射频消融治疗的患者的血清以及 20 例接受诊断性肝活检的患者的对照血清。在射频消融后 1 天，可检测到患者组血清中热休克蛋白 70 水平显著升高。22 例患者中有 9 例观察到两倍以上的升高，且与良好的临床结局相关。对照组无一例患者出现类似的升高。射频消融可导致热休克蛋白 70 释放到血清中，在治疗后 1 天可短暂检测到。升高的热休克蛋白 70 血清水平可能是良好临床结局的生物标志物。
