Department of Pharmacology, School of Medicine, University of California, Irvine, CA 92697-1385, USA.
Pediatr Diabetes. 2011 Aug;12(5):464-72. doi: 10.1111/j.1399-5448.2010.00724.x. Epub 2011 Mar 28.
Obesity (Ob) and type 1 diabetes (T1DM) are associated with increased inflammation and oxidative stress, which are major pathogenetic pathways toward higher cardiovascular risks. Although long-term exercise protects against systemic inflammation and oxidation, acute exercise actually exerts pro-inflammatory and oxidative effects, prompting the necessity for better defining these molecular processes in at-risk patients; in particular, very little is known regarding obese and T1DM children. We therefore examined key inflammatory and oxidative stress variables during exercise in 138 peripubertal children (47 Ob, 12.7 ± 0.4 yr, 22 F, BMI% 97.6 ± 0.2; 49 T1DM, 13.9 ± 0.2 yr, 20 F, body mass index% [BMI] 63.0 ± 3.6; 42 healthy, CL, 13.5 ± 0.5 yr, 24 F, BMI% 57.0 ± 3.6), who performed 10 bouts of 2-min cycling ~80% VO(2max) , separated by 1-min rest intervals. Blood samples were drawn at baseline and peak exercise. Ob displayed elevated baseline interleukin-6 (IL-6, 2.1 ± 0.2 pg/mL, p < 0.005) vs. CL (1.5 ± 0.3), whereas T1DM displayed the greatest maximum exercise-induced change in IL-6 (1.2 ± 0.3) than in both Ob (0.7 ± 0.1, p < 0.001) and CL (0.6 ± 0.1, p < 0.0167). Myeloperoxidase (MPO) was elevated in T1DM (143 ± 30 ng/mL, p < 0.0167) vs. CL (89 ± 10) and Ob (76 ± 6), whereas increases in exercise only occurred in Ob and CL. Disparate baseline and exercise responses were also observed for 8-hydroxy-2'-deoxyguanosine, glutathione, and F(2) -isoprostane. This data show distinct patterns of dysregulation in baseline and adaptive immunologic and oxidative responses to exercise in Ob and T1DM. A full understanding of these alterations is required so that developing exercise regimens aimed at maximizing health benefits for specific dysmetabolic states can be achieved based on complete scientific characterization rather than empirical implementation.
肥胖症（Ob）和 1 型糖尿病（T1DM）与炎症和氧化应激增加有关，这些都是导致心血管风险增加的主要发病途径。尽管长期运动可以预防全身炎症和氧化，但急性运动实际上会产生促炎和氧化作用，这促使我们有必要更好地定义这些高危患者的分子过程；特别是，关于肥胖和 T1DM 儿童的信息知之甚少。因此，我们在 138 名青春期前儿童（47 名肥胖，12.7±0.4 岁，22 名女性，BMI%97.6±0.2；49 名 T1DM，13.9±0.2 岁，20 名女性，体重指数%[BMI]63.0±3.6；42 名健康对照，CL，13.5±0.5 岁，24 名女性，BMI%57.0±3.6）中检查了运动期间的关键炎症和氧化应激变量，这些儿童进行了 10 次 2 分钟的自行车运动，强度约为 80%的 VO2max，每次运动之间休息 1 分钟。在基线和运动峰值时采集血样。与 CL（1.5±0.3）相比，Ob 显示出较高的基础白细胞介素 6（IL-6，2.1±0.2 pg/mL，p<0.005），而 T1DM 显示出最大的运动诱导的 IL-6 变化（1.2±0.3）高于 Ob（0.7±0.1，p<0.001）和 CL（0.6±0.1，p<0.0167）。与 CL（89±10）和 Ob（76±6）相比，T1DM 中髓过氧化物酶（MPO）升高（143±30 ng/mL，p<0.0167），而运动仅在 Ob 和 CL 中引起增加。在基线和运动反应中也观察到 8-羟基-2'-脱氧鸟苷、谷胱甘肽和 F2-异前列腺素的不同模式。这些数据显示了 Ob 和 T1DM 中运动对基线和适应性免疫及氧化反应的失调模式。为了基于全面的科学特征而不是经验实施来制定旨在最大限度地提高特定代谢紊乱状态健康益处的运动方案，需要充分了解这些改变。
