Migliorini Filippo, Eschweiler Jörg, Maffulli Nicola, Hildebrand Frank, Schenker Hanno
Department of Orthopaedic, Trauma and Reconstructive Surgery, RWTH University Hospital, 52074 Aachen, Germany.
Department of Medicine, Surgery and Dentistry, University of Salerno, 84081 Baronissi, Italy.
Life (Basel). 2022 Jun 9;12(6):866. doi: 10.3390/life12060866.
This study investigated the in vivo osseointegration potential of high-performance oxide ceramics (HPOCs) with peptide bone morphogenic protein 2 (BMP-2), comparing them with titanium implants. Histomorphometry was conducted around the distal, proximal, medial, and lateral sides of the implants to quantify the amount of mature and immature ossification within the bone interface. We hypothesised that HPOCs functionalised with BMP-2 promote ossification. HPOCs functionalised with BMP-2 were manufactured at the Department of Dental Materials Science and Biomaterial Research of the RWTH University Aachen, Germany. Histomorphometry was conducted by a professional pathologist in all samples. The region of interest (ROI) represented the percentage of the surrounding area of the implant. The percentages of ROI covered by osteoid implant contact (OIC) and mature bone−implant contact (BIC) were assessed. The surrounding presence of bone resorption, necrosis, and/or inflammation was quantitatively investigated. A total of 36 rabbits were used for the experiments. No bone resorption, necrosis, or inflammation was found in any sample. At the 12-week follow-up, the overall BIC was significantly increased (p < 0.0001). No improvement was evidenced in OIC (p = 0.6). At the 6-week follow-up, the overall OIC was greater in the BMP-2 compared to the titanium group (p = 0.002). The other endpoints of interest evidenced similarity between the two implants at various follow-up time points (p > 0.05). In conclusion, alumina HPOCs functionalised with peptide BMP-2 promote in vivo ossification in a similar fashion to titanium implants.
本研究调查了高性能氧化物陶瓷（HPOCs）与肽骨形态发生蛋白2（BMP-2）在体内的骨整合潜力，并将它们与钛植入物进行比较。在植入物的远侧、近侧、内侧和外侧周围进行组织形态计量学分析，以量化骨界面内成熟和未成熟骨化的量。我们假设用BMP-2功能化的HPOCs能促进骨化。用BMP-2功能化的HPOCs是由德国亚琛工业大学牙体材料科学与生物材料研究部制造的。所有样本均由专业病理学家进行组织形态计量学分析。感兴趣区域（ROI）代表植入物周围区域的百分比。评估类骨质植入物接触（OIC）和成熟骨-植入物接触（BIC）覆盖的ROI百分比。对骨吸收、坏死和/或炎症的周围存在情况进行定量研究。总共36只兔子用于实验。在任何样本中均未发现骨吸收、坏死或炎症。在12周的随访中，总体BIC显著增加（p < 0.0001）。OIC没有改善（p = 0.6）。在6周的随访中，与钛组相比，BMP-2组的总体OIC更大（p = 0.002）。在各个随访时间点，两种植入物的其他感兴趣终点表现出相似性（p > 0.05）。总之，用肽BMP-2功能化的氧化铝HPOCs与钛植入物以相似的方式促进体内骨化。
