Lupo G, Spadaro F, Portera M, Torregrossa M V, Russo Alesi D, Vitale F, Romano N
Istituto di Igiene G. D'Alessandro, Università degli Studi, Palermo, Italy.
Microbiologica. 1988 Oct;11(4):329-37.
21 HIV strains were isolated from peripheral mononuclear cells of 31 adult and pediatric patients with AIDS or ARC and 10 apparently healthy HIV seropositive individuals. Viral isolation rate was correlated to the clinical status of patients, being very high in patients with AIDS or ARC, and lower in apparently healthy individuals. 4 isolates were propagated in cultures of a T-cell continuous line (Molt 4) and their biological properties were compared. The capacity to establish a chronic infection on Molt 4 cells and the extent of cytopathic effect of the infected cultures varied among the different strains. Moreover, differences in endpoint titrations of the supernatants and variable effects on cell multiplication and viability of Molt 4 cells were also observed. Our results further substantiate the existence of a wide heterogeneity of HIV strains.
从31例成年和儿童艾滋病或艾滋病相关综合征（ARC）患者以及10名表面健康的HIV血清阳性个体的外周血单个核细胞中分离出21株HIV毒株。病毒分离率与患者的临床状态相关，在艾滋病或ARC患者中非常高，而在表面健康的个体中较低。4株分离株在T细胞连续系（Molt 4）培养物中传代，并比较了它们的生物学特性。不同毒株在Molt 4细胞上建立慢性感染的能力以及感染培养物的细胞病变效应程度各不相同。此外，还观察到上清液终点滴定的差异以及对Molt 4细胞增殖和活力的不同影响。我们的结果进一步证实了HIV毒株存在广泛的异质性。
