无半胱氨酸天然化学连接逻辑在人甲状旁腺激素（hPTH）合成中的应用。
Application of the logic of cysteine-free native chemical ligation to the synthesis of Human Parathyroid Hormone (hPTH).
机构信息
Bio-Organic Chemistry Laboratory, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
出版信息
Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):5986-9. doi: 10.1073/pnas.1103118108. Epub 2011 Mar 28.
Abstract
The power of chemical synthesis of large cysteine-free polypeptides has been significantly enhanced through the use of nonproteogenic constructs which bear strategically placed thiol groups, enabling native chemical ligation. Central to these much expanded capabilities is the specific, radical-induced, metal-free dethiolation, which can be accomplished in aqueous medium.
摘要
通过使用带有 strategically placed thiol groups 的非天然构建体，显著增强了大半胱氨酸自由多肽的化学合成能力，从而实现了天然化学连接。这些扩展能力的核心是特定的、自由基诱导的、无金属的脱硫反应，可以在水介质中完成。
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