Mariani E, Roda P, Mariani A R, Vitale M, Degrassi A, Papa S, Facchini A
Instituto di Clinica Medica e Gastroenterologia, Istituto di Ricerca Codivilla Putti, Bologna, Italy.
Clin Exp Immunol. 1990 Sep;81(3):479-84. doi: 10.1111/j.1365-2249.1990.tb05359.x.
A cloning technique was used to estimate the frequency of proliferating T cell precursors, the growth capacity of clone-forming cells and the functional activity of clones established in vitro from peripheral blood lymphocytes of young and old people. The mean frequency of proliferating precursors was lower in the elderly as was the proliferative capacity of CD8+ clones. In contrast, CD4+ and CD16+ clones showed a proliferation similar to that obtained from young subjects. When the clones were examined for their functional activity, CD4+ clones from both groups failed to show any cytolytic activity, while CD8+ clones exerted cytolysis against K562 and in antibody-dependent cell-mediated cytotoxicity but this function was reduced in clones derived from old subjects. Similarly, CD16+ clones from the elderly showed a decreased activity at some effector-to-target cell ratios. We conclude that the impaired functional activity (T or NK-dependent) found in the peripheral blood of aged subjects persists after in vitro selection when these cells are analysed at clonal level.
采用克隆技术评估年轻人和老年人外周血淋巴细胞体外培养所建立克隆的增殖性T细胞前体频率、克隆形成细胞的生长能力以及克隆的功能活性。老年人中增殖前体的平均频率较低，CD8 + 克隆的增殖能力也较低。相比之下，CD4 + 和CD16 + 克隆的增殖情况与年轻受试者相似。当检测克隆的功能活性时，两组的CD4 + 克隆均未显示出任何细胞溶解活性，而CD8 + 克隆对K562具有细胞溶解作用，并在抗体依赖性细胞介导的细胞毒性中发挥作用，但老年受试者来源的克隆此功能有所降低。同样，老年受试者的CD16 + 克隆在某些效应细胞与靶细胞比例下活性降低。我们得出结论，当在克隆水平分析这些细胞时，老年受试者外周血中发现的功能活性受损（T细胞或NK细胞依赖性）在体外选择后仍然存在。
