Chrysostomou A, Seshadri R, Morley A A
Scand J Immunol. 1984 Apr;19(4):293-6. doi: 10.1111/j.1365-3083.1984.tb00933.x.
Limiting-dilution cloning of human lymphocytes was used to study cloning and proliferation of lymphocytes in 10 young and 10 elderly individuals. The cloning efficiency for elderly individuals, 3.5%, was significantly lower than that for young individuals, 19.8%. Mixing experiments showed that this decrease was due to both a decrease in the frequency of the clone-forming cells and a decrease in the interacting ability of the lymphocytes. Lymphocytes from elderly individuals which did form clones, however, proliferated at the same rate as did those from young individuals.
采用人淋巴细胞极限稀释克隆法，对10名年轻人和10名老年人的淋巴细胞克隆及增殖情况进行研究。老年人的克隆效率为3.5%，显著低于年轻人的19.8%。混合实验表明，这种降低是由于克隆形成细胞频率的下降以及淋巴细胞相互作用能力的降低所致。然而，来自老年人的确实形成克隆的淋巴细胞，其增殖速度与来自年轻人的淋巴细胞相同。
