Yamauchi Toyohiko, Iwai Hidenao, Yamashita Yutaka
Hamamatsu Photonics K. K., 5000, Hirakuchi, Hamamatsu City, Shizuoka Pref., 434-8601, Japan.
Opt Express. 2011 Mar 14;19(6):5536-50. doi: 10.1364/OE.19.005536.
The subject study demonstrates the imaging of cell activity by quantitatively assessing the motion of intracellular organelles and cell plasma membranes without any contrast agent. The low-coherent interferometric technique and phase-referenced phase shifting technique were integrated to reveal the depth-resolved distribution of intracellular motility. The transversal and vertical spatial resolutions were 0.56 μm and 0.93 μm, respectively, and the mechanical stability of the system was 1.2 nm. The motility of the cell was assessed by mean squared displacement (MSD) and we have compensated for the MSD by applying statistical noise analysis. Thus we show the significant change of intracellular motility after paraformaldehyde treatment in non-labeled cells.
本研究通过在不使用任何造影剂的情况下定量评估细胞内细胞器和细胞质膜的运动来展示细胞活动的成像。将低相干干涉测量技术和相位参考相移技术相结合，以揭示细胞内运动性的深度分辨分布。横向和纵向空间分辨率分别为0.56μm和0.93μm，系统的机械稳定性为1.2nm。通过均方位移（MSD）评估细胞的运动性，并通过应用统计噪声分析对MSD进行了补偿。因此，我们展示了在未标记细胞中多聚甲醛处理后细胞内运动性的显著变化。
