El-Said Waleed Ahmed, Kim Tae-Hyung, Yea Cheol-Heon, Kim Hyuncheol, Choi Jeong-Woo
Interdisciplinary program of Integrated Biotechnology, Sogang University, #1 Shinsu-Dong, Mapo-Gu, Seoul 121-742, Republic of Korea.
J Nanosci Nanotechnol. 2011 Jan;11(1):768-72. doi: 10.1166/jnn.2011.3268.
Alzheimer's disease is a progressive neurodegenerative disorder that is characterized by the deposition of beta-amyloid (Abeta) peptide and the formation of neurofibrillary tangles in neurons. The Abeta peptide is a key molecule in the pathogenesis of Alzheimer's disease and an important marker for early diagnosis. Surface-enhanced Raman scattering (SERS) has recently been attracting keen interest in various fields such as for biosensors or immunoassays. In this study, gold nanoparticles (Au NPs) were electrochemically deposited on an indium tin oxide (ITO) substrate at different heights. Abeta antibodies were immobilized on the Au-NP-coated ITO substrate, after which the interactions between the antigen and the antibody were determined via SERS spectroscopy. The SERS responses were strongest at the Au NP array height of 91 nm, with a good linear relationship that corresponded to the change in the concentration of the antigen. This Au-NP-array-mediated SERS can be applied with a highly sensitive immunodetection biosensor.
阿尔茨海默病是一种进行性神经退行性疾病，其特征在于β-淀粉样蛋白（Aβ）肽的沉积以及神经元中神经原纤维缠结的形成。Aβ肽是阿尔茨海默病发病机制中的关键分子，也是早期诊断的重要标志物。表面增强拉曼散射（SERS）最近在生物传感器或免疫测定等各个领域引起了浓厚兴趣。在本研究中，金纳米颗粒（Au NPs）以不同高度电化学沉积在氧化铟锡（ITO）基底上。将Aβ抗体固定在涂有Au NP的ITO基底上，然后通过SERS光谱法测定抗原与抗体之间的相互作用。在Au NP阵列高度为91 nm时，SERS响应最强，与抗原浓度变化具有良好的线性关系。这种Au-NP阵列介导的SERS可应用于高灵敏度免疫检测生物传感器。
