Chen Liang-Yih, Yang Kuang-Hsuan, Chen Hsiao-Chien, Liu Yu-Chuan, Chen Ching-Hsiang, Chen Qing-Ye
Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan.
Analyst. 2014 Apr 21;139(8):1929-37. doi: 10.1039/c3an02089d.
Surface-enhanced Raman scattering (SERS) utilizing the well-defined localized surface plasmon resonance (LSPR) of Ag and Au nanoparticles (NPs) under resonant irradiation has emerged as a promising spectroscopy technique for providing vibrational information on trace molecules. The Raman scattering intensity from molecules close to the surface of these finely divided metals can be significantly enhanced by a factor of more than 10(6). In addition to the high sensitivity, the reproducibility of the SERS signal is also an important parameter for its reliable application. In this work, we report on the innovative and facile fabrication of a Au NP-decorated SiO2 mask coated on indium tin oxide (ITO) glass as a SERS array substrate. First, a highly ordered porous SiO2 mask with pore sizes of 350 nm in diameter and wall thickness of 60 nm was deposited on ITO glass by using spin coating. Then, Au NPs were controllably decorated into the pores of the conductive ITO glass-bottomed SiO2 mask by using sonoelectrochemical deposition-dissolution cycling (SEDDC). Experimental results indicate that the SERS effect of Rhodamine 6G (R6G) observed on this developed substrate increases with an increase in the deposition time of Au NPs in SEDDC. The corresponding optimal enhancement factor (EF) that is obtained is ca. 6.5 × 10(7). Significantly, this system achieves an optimal reproducibility under a medium-length deposition time of Au NPs in SEDDC with a relative standard deviation (RSD) of 12% for measurements of five spots on different areas. The low RSD of the SERS signal and the large EF suggest that the developed array system can serve as an excellent spectroscopy platform for practical applications in analytical chemistry.
利用银和金纳米颗粒（NPs）在共振辐照下明确的局域表面等离子体共振（LSPR）的表面增强拉曼散射（SERS），已成为一种有前途的光谱技术，可用于提供痕量分子的振动信息。靠近这些细分金属表面的分子的拉曼散射强度可显著增强超过10(6)倍。除了高灵敏度外，SERS信号的重现性也是其可靠应用的一个重要参数。在这项工作中，我们报告了一种创新且简便的方法，用于制备涂覆在氧化铟锡（ITO）玻璃上的金纳米颗粒修饰的二氧化硅掩膜作为SERS阵列基底。首先，通过旋涂在ITO玻璃上沉积孔径为350 nm、壁厚为60 nm的高度有序多孔二氧化硅掩膜。然后，通过超声电化学沉积 - 溶解循环（SEDDC）将金纳米颗粒可控地修饰到导电的ITO玻璃基底二氧化硅掩膜的孔中。实验结果表明，在这种制备的基底上观察到的罗丹明6G（R6G）的SERS效应随着SEDDC中金纳米颗粒沉积时间的增加而增强。获得的相应最佳增强因子（EF）约为6.5×10(7)。值得注意的是，该系统在SEDDC中金纳米颗粒的中等长度沉积时间下实现了最佳重现性，不同区域五个点测量的相对标准偏差（RSD）为12%。SERS信号的低RSD和大EF表明，所开发的阵列系统可作为分析化学实际应用中的优秀光谱平台。
