Department of Microbiology and Immunology, National Cheng Kung University, Tainan, Taiwan.
Biomed Microdevices. 2011 Jun;13(3):585-95. doi: 10.1007/s10544-011-9529-3.
The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 10(1) PFU/ml, which is much better than a conventional ELISA assay (10(3) PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.
酶联免疫吸附测定（ELISA）广泛应用于医学诊断。为了缩短诊断时间，降低 ELISA 检测中样本/试剂的消耗，本研究设计并制作了一种抽吸式、自动、气动驱动的微流控芯片。该微流控芯片集成了多功能微传输/混合单元，用于传输计量和混合样本和试剂，以自动执行整个 ELISA 方案。采用了新的表面修饰方法，实现了高处理能力。登革热病毒的检测灵敏度为 10(1) PFU/ml，明显优于传统 ELISA 检测（10(3) PFU/ml）。整个检测时间仅为 30 分钟，明显快于 96 孔微量滴定板（4 小时）。消耗的样本和试剂体积仅为 12 μl，明显少于传统检测（100 μl）。这种微流控芯片的开发可能在其他免疫传感应用中具有广阔的前景。
