Department of Chemistry, Fudan University, Shanghai, China.
Lab Chip. 2011 Oct 21;11(20):3516-22. doi: 10.1039/c1lc20516a. Epub 2011 Aug 31.
Herein we report fabricating a microfluidic device to monitor harmful algal blooming (HAB). The heterogeneous immuno-enzyme assay was integrated into a self-designed microfluidic chip for rapid and automatic analysis of algal toxins. The device was made from polydimethylsiloxane (PDMS) and was assembled with a home-made control system. The performance of the system was demonstrated by the detection of microcystin, saxitoxin and cylindrospermopsin, the major cyanotoxins. In one single microfluidic chip, multiple samples were controlled and analysed in a parallel manner. Under the optimal conditions, the linear range and the limit of detection of microcystins were 0-5.0 ng mL(-1) and 0.02 ng mL(-1) respectively. The total analysis time was less than 25 min. The designed device was highly automatic, more efficient and economic compared to conventional techniques.
本文报道了一种用于监测有害藻华（HAB）的微流控装置。将异质免疫酶分析集成到自行设计的微流控芯片中，用于快速自动分析藻毒素。该装置由聚二甲基硅氧烷（PDMS）制成，并与自制的控制系统组装在一起。通过检测微囊藻毒素、石房蛤毒素和节旋藻毒素这三种主要的蓝藻毒素来验证系统的性能。在单个微流控芯片中，可并行控制和分析多个样本。在最佳条件下，微囊藻毒素的线性范围和检测限分别为 0-5.0ng/mL 和 0.02ng/mL。整个分析时间不到 25 分钟。与传统技术相比，该设计的装置具有更高的自动化程度、效率和经济性。
