Müller H, Gissmann L
J Gen Virol. 1978 Nov;41(2):315-23. doi: 10.1099/0022-1317-41-2-315.
A virus (MnPV) with the structural characteristics of papilloma viruses was isolated from benign and malignant proliferations of adult animals of the inbred line 'GRA Giessen' of Mastomys natalensis. The particles can be banded in CsCl gradients at densities of 1.34 g/ml (full particles) and 1.29 g/ml (empty particles). The virus DNA has a buoyant density of 1.7104 g/ml and can exist in three different conformations (supercoiled circular, nicked circular and linear), the sedimentation values of which have been determined as 23 to 24S, 16 to 17S and 14 to 15S, respectively. Although the mol. wt. of MnPV DNA is similar to that of HPV 1 DNA, the size of the fragments obtained after cleavage of MnPV DNA with the restriction endonuclease Hae III is quite different from the pattern seen with human papilloma virus. The virion contains 12 different polypeptides; the major structural protein has a mol. wt. of 56 000. MnPV is shown to be the causative agent of the skin proliferations, because tumours can be induced by inoculation of purified virus, whereas no cutaneous alterations are observed when the particles are inoculated in the presence of anti-MnPV serum. MnPV can be re-isolated from the experimentally induced tumours.
从罗非鼠近交系“GRA 吉森”成年动物的良性和恶性增殖组织中分离出一种具有乳头瘤病毒结构特征的病毒（MnPV）。该病毒颗粒在 CsCl 梯度中的浮力密度分别为 1.34 g/ml（完整颗粒）和 1.29 g/ml（空颗粒），可形成条带。病毒 DNA 的浮力密度为 1.7104 g/ml，可存在三种不同构象（超螺旋环状、切口环状和线性），其沉降值分别测定为 23 至 24S、16 至 17S 和 14 至 15S。尽管 MnPV DNA 的分子量与 HPV 1 DNA 相似，但用限制性内切酶 Hae III 切割 MnPV DNA 后得到的片段大小与人乳头瘤病毒的模式有很大不同。病毒粒子含有 12 种不同的多肽；主要结构蛋白的分子量为 56000。MnPV 被证明是皮肤增殖的病原体，因为接种纯化病毒可诱发肿瘤，而在抗 MnPV 血清存在的情况下接种病毒颗粒则未观察到皮肤改变。MnPV 可从实验诱导的肿瘤中重新分离出来。
