Health Promotion and Exercise Program, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku, Tokyo 162-8636, Japan.
Eur J Appl Physiol. 2011 Dec;111(12):2997-3005. doi: 10.1007/s00421-011-1926-z. Epub 2011 Mar 31.
Elevated fasting plasma homocysteine (Hcy) level is a vascular disease risk factor. Plasma Hcy is affected by 5,10-methylenetetrahydofolate reductase (MTHFR) genotype and dietary folate intake. This cross-sectional study in 434 Japanese adults examined the associations among objectively measured physical activity (PA), plasma Hcy adjusting for dietary folate intake, and MTHFR C677T genotype. Daily PA was measured by triaxial accelerometry and all subjects completed a questionnaire about their dietary habits. Plasma Hcy and MTHFR C677T genotype were determined. Plasma Hcy in subjects with the TT genotype was significantly higher than in those with CC or CT genotype (p < 0.001). Plasma Hcy was significantly different between ≥ 200 (7.6 ± 0.2 nmol/mL) and <200 µg/day (8.3 ± 0.3 nmol/mL) folate intake groups (p = 0.003). There were no differences in plasma Hcy adjusting for age, sex, and folate intake between groups according to PA category in all subjects. However, there were significant interactions between time spent in light PA (p = 0.003), vigorous PA (p = 0.001), or inactivity (p = 0.004), and MTHFR genotype. In only the TT genotype, shorter time spent in light PA was associated with higher plasma Hcy than a longer time spent in light PA (11.5 ± 3.3 nmol/mL vs. 8.5 ± 3.3 nmol/mL, p < 0.001), and longer time spent in vigorous PA and inactivity were associated with higher plasma Hcy (11.8 ± 3.3 nmol/mL vs. 8.4 ± 3.2 nmol/mL, 11.6 ± 3.3 nmol/mL vs. 8.4 ± 3.3 nmol/mL, respectively, p < 0.001). In conclusion, light and vigorous PA were associated with plasma Hcy only in the TT genotype, but there were no such associations in all genotypes.
空腹血浆同型半胱氨酸（Hcy）水平升高是血管疾病的危险因素。血浆 Hcy 受到 5,10-亚甲基四氢叶酸还原酶（MTHFR）基因型和饮食叶酸摄入量的影响。本研究对 434 名日本成年人进行了横断面研究，检测了客观测量的体力活动（PA）、调整饮食叶酸摄入量后的血浆 Hcy 以及 MTHFR C677T 基因型之间的关系。每日 PA 通过三轴加速度计测量，所有受试者均完成了有关饮食习惯的问卷。测定血浆 Hcy 和 MTHFR C677T 基因型。TT 基因型受试者的血浆 Hcy 显著高于 CC 或 CT 基因型（p < 0.001）。叶酸摄入量≥200（7.6 ± 0.2 nmol/mL）和<200 µg/天（8.3 ± 0.3 nmol/mL）的两组之间血浆 Hcy 存在显著差异（p = 0.003）。在所有受试者中，根据 PA 类别，调整年龄、性别和叶酸摄入量后，血浆 Hcy 在各组之间没有差异。然而，在轻 PA（p = 0.003）、剧烈 PA（p = 0.001）或不活动（p = 0.004）与 MTHFR 基因型之间存在显著的相互作用。仅在 TT 基因型中，与较长的轻 PA 时间相比，较短的轻 PA 时间与较高的血浆 Hcy 相关（11.5 ± 3.3 nmol/mL 比 8.5 ± 3.3 nmol/mL，p <0.001），较长的剧烈 PA 和不活动时间与较高的血浆 Hcy 相关（11.8 ± 3.3 nmol/mL 比 8.4 ± 3.2 nmol/mL，11.6 ± 3.3 nmol/mL 比 8.4 ± 3.3 nmol/mL，分别，p <0.001）。综上所述，只有 TT 基因型的轻 PA 和剧烈 PA 与血浆 Hcy 相关，但所有基因型均无相关性。
