Schrader Thorsten, Kleine-Ostmann Thomas, Münter Klaus, Jastrow Christian, Schmid Ernst
Physikalisch-Technische Bundesanstalt (PTB), Department 2.2: High Frequency and Electromagnetic Fields, Braunschweig, Germany.
Bioelectromagnetics. 2011 May;32(4):291-301. doi: 10.1002/bem.20634. Epub 2010 Dec 22.
The production of spindle disturbances in a human-hamster hybrid (A(L) ) cell line by an electromagnetic field (EMF) with field strength of 90 V/m at a frequency of 900 MHz was studied in greater detail. The experimental setup presented allows investigating whether either the electrical (E) and/or the magnetic (H) field component of EMF can be associated with the effectiveness of the spindle-disturbing potential. Therefore, both field components of a transversal electromagnetic field (TEM) wave have been separated during exposure of the biological system. This procedure should give more insight on understanding the underlying mechanisms of non-thermal effects of EMF. A statistical comparison of the proportions of the fractions of ana- and telophases with spindle disturbances, obtained for five different exposure conditions with respect to unexposed controls (sham condition), showed that only cells exposed to the H-field component of the EMF were not different from the control. Therefore, the results of the present study indicate that an exposure of cells to EMF at E-field strengths of 45 and 90 V/m, as well as to the separated E component of the EMF, induces significant spindle disturbances in ana- and telophases of the cell cycle.
对人-仓鼠杂交（A(L)）细胞系在900MHz频率下、场强为90V/m的电磁场（EMF）作用下纺锤体紊乱的产生进行了更详细的研究。所展示的实验装置能够探究EMF的电场（E）和/或磁场（H）分量是否与纺锤体干扰潜能的有效性相关。因此，在生物系统暴露期间，横向电磁场（TEM）波的两个场分量已被分离。该过程应能更深入地了解EMF非热效应的潜在机制。对在五种不同暴露条件下相对于未暴露对照（假暴露条件）获得的具有纺锤体干扰的前期和末期细胞比例进行统计比较，结果表明，仅暴露于EMF磁场分量的细胞与对照无差异。因此，本研究结果表明，细胞暴露于场强为45和90V/m的EMF以及EMF分离出的电场分量时，会在细胞周期的前期和末期诱导显著的纺锤体紊乱。
