Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
J Integr Plant Biol. 2009 Apr;51(4):374-81. doi: 10.1111/j.1744-7909.2008.00777.x.
Interactions between an isolate of dark septate endophytes (DSE) and roots of Dendrobium nobile Lindl. seedlings are reported in this paper. The isolate was obtained from orchid mycorrhizas on Dendrobium sp. in subtropical forest. The fungus formed typical orchid mycorrhiza in aseptic co-culture with D. nobile seedlings on modified Murashige-Skoog (MMS) medium. Anatomic observations of the infected roots showed that the DSE hyphae invaded the velamen layer, passed through passage cells in exodermis, entered the cortex cells, and then formed fungal pelotons of orchid mycorrhiza. D. nobile seedlings' plant height, stem diameter, new roots number and biomass were greatly enhanced by inoculating the fungus to seedlings. The fungus was identified as Leptodontidium by sequencing the polymerase chain reaction-amplified rDNA ITS1-5.8S-ITS2 (internal transcribed spacer (ITS)) regions and comparison with similar taxa.
本文报道了一株暗隔内生真菌（DSE）分离株与铁皮石斛（Dendrobium nobile Lindl.）幼苗根系的相互作用。该分离株是从亚热带森林中石斛属植物的兰科菌根中获得的。在改良的 Murashige-Skoog（MMS）培养基上，该真菌与铁皮石斛幼苗进行无菌共培养，形成了典型的兰科菌根。对感染根的解剖观察表明，DSE 菌丝侵入根被层，穿过外皮层的通道细胞，进入皮层细胞，然后形成兰科菌根的真菌聚集体。接种真菌可显著提高铁皮石斛幼苗的株高、茎径、新根数量和生物量。通过对聚合酶链反应扩增的 rDNA ITS1-5.8S-ITS2（内部转录间隔区（ITS））区域进行测序，并与相似分类群进行比较，将该真菌鉴定为 Leptodontidium。
