Huang Hui, Shao Shi-Cheng, Gao Jiang-Yun
Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, the Chinese Academy of Sciences, Mengla 666303, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jun;41(11):2019-2024. doi: 10.4268/cjcmm20161108.
To obtain seedling growth-promoting fungi is a key step in restoration-friendly cultivation of medicinal Dendrobium species, since there are a large number of functionally-unknown endophytic fungi in the roots of Dendrobium plants.In this study, six functionally-unknown endophytic fungal strains were isolated from roots of D.devonianum using single peleton isolation technology, and used in inoculation experiments to test their effectiveness for seedling growth in D.devonianum.After 90 days of inoculation, comparing with the control treatment, FDdS-1, FDdS-2 and FDdS-4 showed strong pathogenic or fatal effects on seedlings; while, FDdS-12, FDdS-9 and FDdS-5 had different effects on seedling growth.FDdS-5 had significant promoting effects on height, fresh and dry weight, stem diameter and root numbers, while FDdS-9 only had significant promoting effect on seedling height, and FDdS-12 had a negative effect on seedling growth.According to the anatomical features of the inoculated roots, FDdS-5 fungi could infect the velamina of seedlings and the existence of symbiosis pelotons in the cortex cells, suggesting that FDdS-5 is a mycorrhiza fungi of D.devonianum.FDdS-5 and FDdS-9 were identified as Sebacina vermifera and Sebacina sp.by molecular technologies.By using FDdS-5 in the restoration-friendly cultivation of D.devonianum, it could effectively promote seedling growth and shorten the seedling growth periods.The results will aid in reintroduction and cultivation of D.devonianum.
获得促幼苗生长真菌是药用石斛物种恢复友好型栽培的关键步骤，因为石斛属植物根中有大量功能未知的内生真菌。在本研究中，采用单菌根分离技术从云南石仙桃的根中分离出6株功能未知的内生真菌菌株，并用于接种实验，以测试它们对云南石仙桃幼苗生长的有效性。接种90天后，与对照处理相比，FDdS - 1、FDdS - 2和FDdS - 4对幼苗表现出强烈的致病或致死作用；而FDdS - 12、FDdS - 9和FDdS - 5对幼苗生长有不同影响。FDdS - 5对株高、鲜重和干重、茎直径和根数有显著促进作用，而FDdS - 9仅对幼苗株高有显著促进作用，FDdS - 12对幼苗生长有负面影响。根据接种根的解剖特征，FDdS - 5真菌可感染幼苗的根被，皮层细胞中存在共生菌根圈，表明FDdS - 5是云南石仙桃的一种菌根真菌。通过分子技术将FDdS - 5和FDdS - 9鉴定为蚯蚓状角担菌和角担菌属。在云南石仙桃的恢复友好型栽培中使用FDdS - 5，可有效促进幼苗生长并缩短幼苗生长期。研究结果将有助于云南石仙桃的迁地保育和栽培。
