光周期影响培养的绿藻巴氏杜氏藻中的内源性吲哚胺。
Photoperiod influences endogenous indoleamines in cultured green alga Dunaliella bardawil.
作者信息
Ramakrishna A, Dayananda C, Giridhar P, Rajasekaran T, Ravishankar G A
机构信息
Plant Cell Biotechnology Department, Central Food Technological Research Institute (Council of Scientific and Industrial Research), Mysore 570 020, India.
出版信息
Indian J Exp Biol. 2011 Mar;49(3):234-40.
Effect of light intensity and photoperiod on growth, indoleamines and carotenoid production was studied in unicellular green algae D. bardawil. Maximum biomass and carotenoid contents were found when cultures were grown in light (intensity of 2.0 Klux) at a photoperiod of 16/8h light and dark cycle. There was a profound influence of tested photoperiod conditions of light:dark viz. 8:16, 10:14, and 12:12 hr, continuous light on indoleamines (SER and MEL) production as estimated by HPLC and confirmed by mass spectral data obtained from LC-MS-ESI studies. Serotonin level increased from 908 to 1765 pg/g fresh wt with increase in light duration and melatonin level increased from 267 to 584 pg/g fresh wt during increase in dark phase. Carotenoids production was high in continuous light than other tested conditions.
研究了光照强度和光周期对单细胞绿藻巴达维亚杜氏藻（D. bardawil）生长、吲哚胺和类胡萝卜素产生的影响。当培养物在光照强度为2.0千勒克斯、光周期为16/8小时明暗循环的条件下生长时，发现生物量和类胡萝卜素含量最高。通过高效液相色谱法（HPLC）估计并经液相色谱-质谱-电喷雾电离（LC-MS-ESI）研究获得的质谱数据证实，所测试的光暗光周期条件（即8:16、10:14和12:12小时、连续光照）对吲哚胺（血清素和褪黑素）的产生有深远影响。随着光照时间增加，血清素水平从908皮克/克鲜重增加到1765皮克/克鲜重，在黑暗阶段增加期间，褪黑素水平从267皮克/克鲜重增加到584皮克/克鲜重。连续光照条件下类胡萝卜素的产量高于其他测试条件。
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