TASC, Inc., Biomedical Sciences and Technology Department, San Antonio, Texas 78235, USA.
J Biomed Opt. 2011 Mar;16(3):036003. doi: 10.1117/1.3548881.
We measured threshold temperatures for cell death resulting from short (0.1-1.0 s) 514-nm laser exposures using an in vitro retinal model. Real-time thermal imaging at sub-cellular resolution provides temperature information that is spatially correlated with cells at the boundary of cell death, as indicate by post-exposure fluorescence images. Our measurements indicate markedly similar temperatures, not only around individual boundaries (single exposure), but among all exposures of the same duration in a laser irradiance-independent fashion. Two different methods yield similar threshold temperatures with low variance. Considering the experimental uncertainties associated with the thermal camera, an average peak temperature of 53 ± 2 °C is found for laser exposures of 0.1, 0.25, and 1.0 s. Additionally, we find a linear relationship between laser exposure duration and time-averaged integrated temperature. The mean thermal profiles for cells at the boundary of death were assessed using the Arrhenius rate law using parameter sets (frequency factor and energy of activation) found in three different articles.
我们使用体外视网膜模型测量了由短（0.1-1.0 s）514nm 激光照射引起的细胞死亡的阈值温度。实时亚细胞分辨率热成像提供了与细胞死亡边界处细胞空间相关的温度信息，这是通过照射后的荧光图像来指示的。我们的测量结果表明，不仅在单个边界周围（单次照射），而且在以激光辐照度无关的方式照射相同持续时间的所有照射中，温度都非常相似。两种不同的方法以低方差产生相似的阈值温度。考虑到与热像仪相关的实验不确定性，对于 0.1、0.25 和 1.0 s 的激光照射，平均峰值温度为 53 ± 2°C。此外，我们发现激光照射持续时间和时间平均积分温度之间存在线性关系。使用在三篇不同文章中找到的参数集（频率因子和激活能），使用阿累尼乌斯速率定律评估了死亡边界处细胞的平均热分布。
