University of California, Irvine, Beckman Laser Institute, Irvine, California 92612, USA.
J Biomed Opt. 2011 Mar;16(3):036010. doi: 10.1117/1.3555180.
A fiber-based multimodal imaging system that combines multiphoton microscopy (MPM) with Fourier domain optical coherence microscopy (OCM) is reported. The system uses a fiber-based femtosecond laser, a fiber coupler, and a double-clad fiber (DCF) device. The fiber laser has a central wavelength of 1.04 μm and bandwidth of 29 nm. Longer excitation wavelength is used to increase penetration depth and increase the excitation efficiency for dyes, such as red fluorescent dyes. A single mode fiber coupler is used to replace the free-space beam splitter and one arm of the coupler is fused with a double-clad fiber device. The MPM and OCM share the same excitation light path in the core of a double-clad fiber, while the OCM and MPM signals were collected by the core and clad of the double-clad fiber, respectively. The performance of the introduced double-clad device is analyzed. The device can confine all the excitation light in the core and has a collection efficiency of 20% for the MPM signal. The efficiency can be further increased by fusing more multimode fibers with the DCF. Simultaneous optical coherence microscopic imaging, second harmonic generation imaging, and two-photon excitation fluorescence imaging are demonstrated in biological samples.
一种基于光纤的多模态成像系统，将多光子显微镜（MPM）与傅里叶域光学相干显微镜（OCM）相结合，该系统使用基于光纤的飞秒激光器、光纤耦合器和双包层光纤（DCF）器件。光纤激光器的中心波长为 1.04μm，带宽为 29nm。使用较长的激发波长来增加穿透深度，并提高染料（如红色荧光染料）的激发效率。单模光纤耦合器用于替代自由空间分束器，并且耦合器的一个臂与双包层光纤器件熔接。MPM 和 OCM 在双包层光纤的纤芯中共享相同的激发光路，而 OCM 和 MPM 信号分别由双包层光纤的纤芯和包层收集。分析了所介绍的双包层器件的性能。该器件可以将所有激发光限制在纤芯内，并且对 MPM 信号的收集效率为 20%。通过与更多的多模光纤熔接，可以进一步提高效率。在生物样本中演示了同时光学相干微观成像、二次谐波产生成像和双光子激发荧光成像。
