Shanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, National Tissue Engineering Center of China, Shanghai, China.
Stem Cells Dev. 2012 Jan;21(1):143-51. doi: 10.1089/scd.2010.0585. Epub 2011 May 12.
Human parthenogenetic embryonic stem cells (hpESCs) established from artificially activated oocytes have a wider immune-matching ability because of their homozygosity in the major histocompatibility complex alleles. Whether these cells possess the differentiation capacity similar to regular human embryonic stem cells (hESCs) derived from fertilized eggs is unclear. The aims of this study were to determine whether hpESCs could be differentiated into multipotent mesenchymal stem cell (MSC)-like cells in vitro and then compare these cells with those derived from hESCs. MSC-like cells were obtained from both hpESCs and hESCs, which exhibited similar cell surface marker expression profiles. Further analyses revealed that cells derived from hpESCs possessed stronger osteogenic but weaker adipogenic potentials compared with cells derived from hESCs. This is the first work that demonstrates the differentiation of hpESCs into multipotent MSC-like cells. These hpESCs could be a potential source for cell-based therapies.
人孤雌胚胎干细胞（hpESCs）来源于人工激活的卵母细胞，由于其主要组织相容性复合体等位基因的纯合性，具有更广泛的免疫匹配能力。这些细胞是否具有与常规受精胚胎来源的人类胚胎干细胞（hESCs）相似的分化能力尚不清楚。本研究旨在确定 hpESCs 是否可以在体外分化为多能间充质干细胞（MSC）样细胞，然后将这些细胞与 hESCs 衍生的细胞进行比较。MSC 样细胞分别从 hpESCs 和 hESCs 中获得，它们表现出相似的细胞表面标志物表达谱。进一步的分析表明，与 hESCs 衍生的细胞相比，hpESCs 衍生的细胞具有更强的成骨能力，但成脂能力较弱。这是首次证明 hpESCs 分化为多能 MSC 样细胞的工作。这些 hpESCs 可能是细胞治疗的潜在来源。
