Toxins and stress in fish: proteomic analyses and response network.

Fish models are increasingly used in toxicological studies in the laboratory as well as in the field. In addition to contributing to the analysis of toxicity mechanisms, one major aim is to select biomarkers from among the metabolic responses to toxic agents observed that could be useful for surveying the aquatic environment. Since proteomics is a developing field in toxicological research, it seems opportune to explore the data obtained using this approach. This article proposes an overview of proteomic studies of fish exposed to environmental stressors comprising a cyanotoxin and the response networks observed. We tend to take a broad view of how proteins communicate and function within the cell, often encompassing large numbers of proteins that operate in pathways. We start by presenting and discussing the data from four experiments in which the medaka fish was treated under the same conditions with the cyanotoxin, microcystin-LR (MC-LR). Liver proteins were analyzed using two techniques: 2D electrophoresis and LCMSMS. In the second and main part of our paper, the proteomic data obtained from fish contaminated with chemicals, including those reported above concerning the medaka fish intoxicated with MC-LR, are considered in the round in order to identify fish responses to chemical stress. A tentative general overview of how groups of proteins work together depending on exposure and/or subcellular location is proposed, with the inclusion of MC-LR data obtained in mice for comparison.