Department of Chemistry, University of New Orleans, New Orleans, LA 70148, USA.
Integr Comp Biol. 2012 Nov;52(5):626-35. doi: 10.1093/icb/ics063. Epub 2012 Apr 25.
The Gulf killifish, Fundulus grandis, is a small teleost fish that inhabits marshes of the Gulf of Mexico and demonstrates high tolerance of environmental variation, making it an excellent subject for the study of physiological and molecular adaptations to environmental stress. In the present study, two-dimensional (2D) gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry were used to resolve and identify proteins from five tissues: skeletal muscle, liver, brain, heart, and gill. Of 864 protein features excised from 2D gels, 424 proteins were identified, corresponding to a 49% identification rate. For any given tissue, several protein features were identified as the same protein, resulting in a total of 254 nonredundant proteins. These nonredundant proteins were categorized into a total of 11 molecular functions, including catalytic activity, structural molecule, binding, and transport. In all tissues, catalytic activity and binding were the most highly represented molecular functions. Comparing across the tissues, proteome coverage was lowest in skeletal muscle, due to a combination of a low number of gel spots excised for analysis and a high redundancy of identifications among these spots. Nevertheless, the identification of a substantial number of proteins with high statistical confidence from other tissues suggests that F. grandis may serve as a model fish for future studies of environmental proteomics and ultimately help to elucidate proteomic responses of fish and other vertebrates to environmental stress.
海湾金沙鲷（Fundulus grandis）是一种小型硬骨鱼，栖息于墨西哥湾的沼泽地，对环境变化具有很强的耐受性，是研究生理和分子适应环境胁迫的理想对象。本研究采用二维凝胶电泳（2DGE）和基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF-MS）技术，从 5 种组织（骨骼肌、肝脏、脑、心脏和鳃）中分离和鉴定蛋白质。从 2D 凝胶中切取 864 个蛋白质点，鉴定到 424 个蛋白，鉴定率为 49%。对于任意给定的组织，有几个蛋白质点被鉴定为同一种蛋白质，共鉴定到 254 个非冗余蛋白。这些非冗余蛋白共分为 11 种分子功能，包括催化活性、结构分子、结合和转运。在所有组织中，催化活性和结合是最主要的分子功能。比较不同组织的蛋白质组覆盖度，骨骼肌的蛋白质组覆盖度最低，这是由于分析中切取的凝胶斑点数量较少，以及这些斑点中的鉴定结果高度重复所致。然而，从其他组织中鉴定到了大量具有高统计可信度的蛋白质，这表明海湾金沙鲷可能成为未来环境蛋白质组学研究的模式鱼类，并最终有助于阐明鱼类和其他脊椎动物对环境胁迫的蛋白质组响应。
