Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland.
Res Vet Sci. 2011 Dec;91(3):e163-8. doi: 10.1016/j.rvsc.2011.03.007. Epub 2011 Mar 31.
The Toggenburg orbivirus (TOV), a recently discovered virus related to bluetongue virus (BTV), has been identified in goats in Switzerland, Italy and Germany. Isolation of TOV in vitro has not yet been achieved and the transmission mechanisms are still unknown. In the experimental infection of pregnant goats described here, TOV could not be detected in secretion/excretion samples or fetal blood. Material from the goat experiment was used as inoculum for propagating the virus in vitro. To enhance the infectivity of TOV several modified protocols, e.g. pretreatment of the virus with trypsin, polyethylene glycol-mediated infection and lipofection were applied. Isolation of TOV, attempts to infect Culicoides nubeculosus by feeding TOV-positive blood and intracerebral inoculation of newborn mice were unsuccessful. The results of these studies suggest that TOV requires specific but different factors than other BTVs for infection and replication outside of its natural caprine host.
托高恩包柔体病毒（Toggenburg orbivirus，TOV）是一种最近发现的与蓝舌病病毒（ bluetongue virus，BTV）相关的病毒，已在瑞士、意大利和德国的山羊中被鉴定出来。目前尚未在体外成功分离出 TOV，其传播机制仍不清楚。在本研究中对怀孕山羊进行的实验性感染表明，在分泌/排泄物样本或胎血中均无法检测到 TOV。使用来自山羊实验的材料作为接种物在体外繁殖病毒。为了提高 TOV 的感染力，应用了几种改良的方案，例如用胰蛋白酶预处理病毒、聚乙二醇介导的感染和脂质体转染。未能成功分离 TOV，也未能通过喂食 TOV 阳性血液感染库蠓属（Culicoides nubeculosus）或通过脑内接种新生小鼠来感染。这些研究结果表明，TOV 感染和复制所需的特定因素不同于其天然宿主山羊中的其他 BTV。
