Department of Biology, Ferdowsi University of Mashhad, Iran.
Fitoterapia. 2011 Jul;82(5):742-9. doi: 10.1016/j.fitote.2011.03.005. Epub 2011 Mar 31.
The aim of this study was to investigate the cytotoxic and anticancer activities of stylosin, a monoterpene extracted from an edible plant, Ferula ovina, on 5637 and HFF3 cells using MTT and comet assays and DAPI staining. To assess stylosin effects, cells were cultured in the presence of various concentrations of stylosin during three days; the IC(50) of stylosin on cancerous 5637 cells was less than its value on HFF3 normal cells, indicating that it might have anticancer properties. Investigating the mechanism of stylosin action revealed that it quickly induced DNA lesions and increased the number of apoptotic cells.
本研究旨在通过 MTT 和彗星试验和 DAPI 染色法，研究从食用植物欧芹中提取的单萜stilosin 对 5637 和 HFF3 细胞的细胞毒性和抗癌活性。为了评估 stilosin 的作用，将细胞在含有不同浓度 stilosin 的条件下培养三天；stilosin 对癌细胞 5637 的 IC50 小于其对正常细胞 HFF3 的 IC50，表明它可能具有抗癌特性。研究 stilosin 作用的机制表明，它能迅速诱导 DNA 损伤并增加凋亡细胞的数量。
