Tazik Zahra, Rahnama Kamran, White James Francis, Soltanloo Hassan, Hasanpour Maede, Iranshahi Mehrdad
Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Department of Biology, Rutgers University, New Brunswick, New Jersey, U.S.A.
Iran J Basic Med Sci. 2020 Dec;23(12):1565-1570. doi: 10.22038/ijbms.2020.46334.10703.
is an Iranian medicinal plant. Tschimgine and stylosin are two of its major monoterpene derivatives. In this study, we proceeded to investigate some fungal endophytes from that can produce plant secondary metabolites.
The isolated endophytic fungi were fermented in potato dextrose broth (PDB) medium and their extracts were screened for the presence of the plant compounds by liquid chromatography-tandem mass spectrometry (LC-MS). Endophytes identification was performed by morphological and molecular methods. Three markers (ITS, LSU, and TEF1) were used for accurate molecular identification.
Forty isolates from 9 different genera of endophytic fungi were identified, of which two recently reported species of and were able to produce tschimgine and stylosin.
These fungi can be used as a substitute for the production of plant's medicinal compounds independent of wild populations of the source plant.
是一种伊朗药用植物。tschimgine和异土木香内酯是其两种主要的单萜衍生物。在本研究中，我们着手研究来自该植物的一些能够产生植物次生代谢产物的真菌内生菌。
将分离得到的内生真菌在马铃薯葡萄糖肉汤（PDB）培养基中发酵，并用液相色谱 - 串联质谱（LC - MS）对其提取物中植物化合物的存在情况进行筛选。通过形态学和分子方法对内生菌进行鉴定。使用三个标记（ITS、LSU和TEF1）进行准确的分子鉴定。
鉴定出9个不同属的40株内生真菌分离株，其中两个最近报道的物种能够产生tschimgine和异土木香内酯。
这些真菌可作为生产该植物药用化合物的替代品，而无需依赖源植物的野生种群。
