Biotechnology Research Center and School of Pharmacy, Mashhad University of Medical Sciences, 91775-1365, Mashhad, Iran.
Daru. 2013 May 23;21(1):39. doi: 10.1186/2008-2231-21-39.
Ferula species are reputed in folk medicine for the treatment of a variety of disorders. There have been sporadic reports on the chemopreventive and chemosensitizing activities of some terpenoid coumarin derivatives from the genus Ferula. The present study investigated the cytotoxic activity of 11 phytochemicals (conferone, farnesiferol A, acantrifoside E, mogoltadone, diversin, galbanic acid, herniarin, 7-isopentenyloxycoumarin, umbelliprenin, stylosin and tschimgine) from Ferula species together with a newly synthesized prenylated derivative of curcumin (gercumin II).
Cytotoxic activity of phytochemicals was evaluated against ovarian carcinoma (CH1), lung cancer (A549) and melanoma (SK-MEL-28) cell lines using MTT assay.
Overall, moderate cytotoxic activity was observed from the tested compounds with IC50 values in the micromolar range. The highest activity against CH1 and A549 lines was from conferone while stylosin and tschimgine were the most potent compounds against SK-MEL-28 line. In conclusion, the findings of the present investigation did not support a potent cytotoxic activity of the tested phytochemicals against CH1, A549 and SK-MEL-28 cell lines. With respect to previous reports, the beneficial impact of these phytochemicals in cancer therapy may be more attributable to their chemopreventive or chemosensitizing activity rather than direct cytotoxic effects.
在民间医学中，阿魏属植物被认为可治疗多种疾病。一些倍半萜香豆素衍生物具有化学预防和化学增敏活性，有关其的零星报道已有出现。本研究调查了 11 种植物化学物质（conferone、farnesiferol A、acantrifoside E、mogoltadone、diversin、galbanic acid、herniarin、7-异戊烯氧基香豆素、umbelliprenin、stylosin 和 tschimgine）以及一种新合成的姜黄素的类异戊二烯基衍生物（gercumin II）对阿魏属植物的细胞毒性活性。
用 MTT 法评估植物化学物质对卵巢癌（CH1）、肺癌（A549）和黑色素瘤（SK-MEL-28）细胞系的细胞毒性活性。
总的来说，从测试的化合物中观察到中等的细胞毒性活性，IC50 值在微摩尔范围内。conferone 对 CH1 和 A549 系的活性最高，而 stylosin 和 tschimgine 对 SK-MEL-28 系的活性最强。综上所述，本研究的结果不支持测试的植物化学物质对 CH1、A549 和 SK-MEL-28 细胞系具有强烈的细胞毒性活性。与之前的报道相比，这些植物化学物质在癌症治疗中的有益影响可能更多归因于其化学预防或化学增敏活性，而不是直接的细胞毒性作用。
