Handali Sukwan, Klarman Molly, Gaspard Amanda N, Noh John, Lee Yeuk-Mui, Rodriguez Silvia, Gonzalez Armando E, Garcia Hector H, Gilman Robert H, Tsang Victor C W, Wilkins Patricia P
Division of Parasitic Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, 4770 Buford Highway, Chamblee, GA 30341, USA.
Clin Vaccine Immunol. 2010 Jan;17(1):68-72. doi: 10.1128/CVI.00339-09. Epub 2009 Nov 11.
One of the best-characterized tests for the diagnosis of neurocysticercosis is the enzyme-linked immunoelectrotransfer blot assay, developed at the CDC, which uses lentil lectin-purified glycoproteins (LLGPs) extracted from Taenia solium cysticerci. The purification of the LLGP antigens has been difficult to standardize, and the polyacrylamide gel system used for the immunoblot assay is not easily transferable to other laboratories. In this study, we developed a multiantigen printing immunoassay (MAPIA) to compare the performance of multiple recombinant Taenia solium proteins with the potential for the detection of cysticercosis and taeniasis. We prepared MAPIA strips using six cysticercosis and two taeniasis diagnostic proteins and compared the performance of the proteins with sera collected from defined cysticercosis and taeniasis cases. Of the six cysticercosis antigens, rT24H performed well in detecting cases with two or more viable cysts in the brain (sensitivity and specificity, 97% and 99.4%, respectively); the use of a combination of cysticercosis antigens did not improve the sensitivity of the test and decreased the specificity. None of the antigens could differentiate the different clinical presentations of cysticercosis. Both of the taeniasis antigens (rES33 and rES38) had the same sensitivity of 99.4% and specificities of 93.9% and 94.5%, respectively. Some cross-reactivity against rES33 and rES38 was found, especially with sera from cases infected with Schistosoma mansoni. We conclude that MAPIA is a simple and effective tool that may be used to compare antibody responses to different cysticercosis and taeniasis antigens and, in this case, may be useful for the rapid detection of T. solium cases.
用于诊断神经囊尾蚴病的最佳特征性检测方法之一是疾病控制与预防中心（CDC）开发的酶联免疫电转移印迹分析，该方法使用从猪带绦虫囊尾蚴中提取的扁豆凝集素纯化糖蛋白（LLGPs）。LLGP抗原的纯化难以标准化，且用于免疫印迹分析的聚丙烯酰胺凝胶系统不易转移到其他实验室。在本研究中，我们开发了一种多抗原印迹免疫分析（MAPIA），以比较多种重组猪带绦虫蛋白在检测囊尾蚴病和绦虫病方面的性能。我们使用六种囊尾蚴病诊断蛋白和两种绦虫病诊断蛋白制备了MAPIA试纸条，并将这些蛋白与从明确的囊尾蚴病和绦虫病病例中采集的血清进行性能比较。在六种囊尾蚴病抗原中，rT24H在检测脑内有两个或更多活囊的病例时表现良好（敏感性和特异性分别为97%和99.4%）；联合使用囊尾蚴病抗原并未提高检测的敏感性，反而降低了特异性。没有一种抗原能够区分囊尾蚴病的不同临床表现。两种绦虫病抗原（rES33和rES38）的敏感性均为99.4%，特异性分别为93.9%和94.5%。发现rES33和rES38存在一些交叉反应，尤其是与曼氏血吸虫感染病例的血清。我们得出结论，MAPIA是一种简单有效的工具，可用于比较对不同囊尾蚴病和绦虫病抗原的抗体反应，在这种情况下，可能有助于快速检测猪带绦虫病例。
