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pmoA 引物用于检测厌氧甲烷营养菌。

pmoA Primers for detection of anaerobic methanotrophs.

机构信息

Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, Netherlands.

出版信息

Appl Environ Microbiol. 2011 Jun;77(11):3877-80. doi: 10.1128/AEM.02960-10. Epub 2011 Apr 1.

DOI:10.1128/AEM.02960-10
PMID:21460105
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3127593/
Abstract

Published pmoA primers do not match the pmoA sequence of "Candidatus Methylomirabilis oxyfera," a bacterium that performs nitrite-dependent anaerobic methane oxidation. Therefore, new pmoA primers for the detection of "Ca. Methylomirabilis oxyfera"-like methanotrophs were developed and successfully tested on freshwater samples from different habitats. These primers expand existing molecular tools for the study of methanotrophs in the environment.

摘要

已发表的 pmoA 引物与进行亚硝酸盐依赖型厌氧甲烷氧化的细菌“Candidatus Methylomirabilis oxyfera”的 pmoA 序列不匹配。因此，开发了用于检测“Ca. Methylomirabilis oxyfera”-样甲烷营养菌的新 pmoA 引物，并在来自不同生境的淡水样本中成功进行了测试。这些引物扩展了现有的分子工具，可用于研究环境中的甲烷营养菌。

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