State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
CGMCC - China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):129-133. doi: 10.1099/ijs.0.038091-0. Epub 2012 Feb 17.
Two Gram-positive, rod-shaped, non-spore-forming bacteria (strains RHLT(1)-17(T) and RHLT(2)-1(T)) were isolated from Hailuogou glacier in Szechwan province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belonged to the genus Nocardioides and shared 97.8 % sequence similarity with each other and 97.6 and 98.4 % sequence similarity, respectively, with Nocardioides kribbensis KSL-2(T). Strain RHLT(1)-17(T) grew at 0-35 °C and strain RHLT(2)-1(T) grew at 0-25 °C. The major cellular fatty acids of strain RHLT(1)-17(T) were C(17 : 1)ω8c (32.69 %) and iso-C(16 : 0) (21.74 %). The major cellular fatty acids of strain RHLT(2)-1(T) were C(18 : 1)ω9c (28.72 %), summed feature 3 (17.14 %; comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c), iso-C(16 : 0) (14.35 %), C(16 : 0) (9.96 %) and iso-C(14 : 0) (8.34 %). Both strains contained ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H(4)) as the predominant menaquinone. On the basis of data obtained using a polyphasic approach, two novel species, Nocardioides szechwanensis sp. nov. (type strain RHLT(1)-17(T) = CGMCC 1.11147(T) = NBRC 108562(T)) and Nocardioides psychrotolerans sp. nov. (type strain RHLT(2)-1(T) =CGMCC 1.11156(T) = NBRC 108563(T)), are proposed.
从中国四川省海螺沟冰川中分离到两株革兰氏阳性、杆状、非孢子形成细菌（RHLT(1)-17(T)和 RHLT(2)-1(T)株）。基于 16S rRNA 基因序列的系统发育分析表明，这些菌株属于诺卡氏菌属，彼此之间的序列相似度为 97.8%，与 Nocardioides kribbensis KSL-2(T)的序列相似度分别为 97.6%和 98.4%。RHLT(1)-17(T)株在 0-35°C 下生长，RHLT(2)-1(T)株在 0-25°C 下生长。RHLT(1)-17(T)株的主要细胞脂肪酸为 C(17 : 1)ω8c（32.69%）和 iso-C(16 : 0)（21.74%）。RHLT(2)-1(T)株的主要细胞脂肪酸为 C(18 : 1)ω9c（28.72%），summed feature 3（由 C(16 : 1)ω7c 和/或 C(16 : 1)ω6c 组成，17.14%），iso-C(16 : 0)（14.35%），C(16 : 0)（9.96%）和 iso-C(14 : 0)（8.34%）。两株菌的细胞壁肽聚糖中均含有 ll-2,6-二氨基庚二酸，主要的menaquinone 为 MK-8(H(4))。基于多相分类研究获得的数据，提出了两个新种，即四川诺卡氏菌（模式菌株 RHLT(1)-17(T) = CGMCC 1.11147(T) = NBRC 108562(T)）和耐冷诺卡氏菌（模式菌株 RHLT(2)-1(T) =CGMCC 1.11156(T) = NBRC 108563(T)）。
