Departamento de Microbiología y Ecología, Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, 46980 Paterna (Valencia), Spain.
Istituto di Chimica Biomolecolare, CNR, Pozzuoli, Italy.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):438-444. doi: 10.1099/ijs.0.029447-0. Epub 2011 Apr 1.
Strains 2SM5(T) and 2SM6, two strictly aerobic chemo-organotrophic gammaproteobacteria, were isolated from Mediterranean seawater off the coast of Vinaroz, Castellón, Spain, in February, 1990. They were extensively characterized by a polyphasic study that placed them in the genus Pseudomonas. Phylogenetic analysis of 16S rRNA gene sequences showed that both strains shared 100 % sequence similarity and were closely related to members of the Pseudomonas pertucinogena clade, with less than 97.3 % similarity to strains of established species; Pseudomonas xiamenensis was the closest relative. Analysis of sequences of three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the phylogenetic assignment of the Mediterranean isolates. Chemotaxonomic traits such as quinone and polar lipid composition also corroborated the placement of strains 2SM5(T) and 2SM6 in the gammaproteobacteria. Other phenotypic traits, including fatty acid composition, enabled clear differentiation of both isolates from other species of Pseudomonas. We therefore conclude that strains 2SM5(T) and 2SM6 represent a novel species of Pseudomonas, for which the name Pseudomonas litoralis is proposed; the type strain is 2SM5(T) ( = CECT 7670(T) = KCTC 23093(T)).
菌株 2SM5(T) 和 2SM6 是两株严格需氧的化能有机营养型γ-变形菌，于 1990 年 2 月从西班牙卡斯特利翁省比那罗斯沿海的地中海海水中分离得到。通过多相研究对它们进行了广泛的表征，将它们归入假单胞菌属。16S rRNA 基因序列的系统发育分析表明，两株菌的序列相似度为 100%，与假单胞菌 pertucinogena 分支的成员密切相关，与已建立的种的菌株相似度小于 97.3%；与厦门假单胞菌最为接近。三个管家基因（rpoB、rpoD 和 gyrB）序列分析进一步证实了地中海分离株的系统发育归属。类脂醌和极性脂组成等化学分类学特征也支持菌株 2SM5(T) 和 2SM6 归属于γ-变形菌。其他表型特征，包括脂肪酸组成，使这两个分离株能够与其他假单胞菌种明显区分开来。因此，我们得出结论，菌株 2SM5(T) 和 2SM6 代表了假单胞菌属的一个新种，建议将其命名为海栖假单胞菌；其模式菌株为 2SM5(T)（=CECT 7670(T)=KCTC 23093(T)）。
