Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):409-413. doi: 10.1099/ijs.0.030106-0. Epub 2011 Apr 1.
A gram-negative, pink-pigmented, non-motile, strictly aerobic rod, designated CNU040(T), was isolated from seawater from the coast of Jeju Island in Korea. The temperature, pH and NaCl ranges for growth were 4-30 °C, pH 5.5-10.0 and 0-5.0 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CNU040(T) belonged to a distinct lineage in the genus Algoriphagus and exhibited high sequence similarity with Algoriphagus terrigena DS-44(T) (98.3 %) and Algoriphagus alkaliphilus AC-74(T) (96.6 %) and lower sequence similarity (<96.0 %) with all other members of the genus Algoriphagus. DNA-DNA relatedness between strain CNU040(T) and A. terrigena KCTC 12545(T) was 44.5 %. The DNA G+C content of the isolate was 48.5 mol% and the major respiratory quinone was menaquinone-7. The major cellular fatty acids were iso-C(15 : 0) (28.6 %) and summed feature 3 (consisting of iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c; 24.0 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown amino lipid, one unknown aminophospholipid and three unknown polar lipids. On the basis of phenotypic, phylogenetic and genotypic data, strain CNU040(T) represents a novel species within the genus Algoriphagus, for which the name Algoriphagus jejuensis sp. nov. is proposed. The type strain is CNU040(T) ( = KCTC 22647(T) = JCM 16112(T)).
一株革兰氏阴性、粉红色、非运动、严格需氧的棒状菌，命名为 CNU040(T)，从韩国济州岛海岸的海水中分离得到。该菌的最适生长温度、pH 值和 NaCl 范围分别为 4-30°C、pH5.5-10.0 和 0-5.0%NaCl。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CNU040(T)属于 Algoriphagus 属的一个独特谱系，与 Algoriphagus terrigena DS-44(T)（98.3%）和 Algoriphagus alkaliphilus AC-74(T)（96.6%）具有较高的序列相似性，与 Algoriphagus 属的其他所有成员的序列相似性较低（<96.0%）。菌株 CNU040(T)与 A. terrigena KCTC 12545(T)之间的 DNA-DNA 同源性为 44.5%。该分离株的 DNA G+C 含量为 48.5mol%，主要呼吸醌为menaquinone-7。主要细胞脂肪酸为 iso-C(15：0)（28.6%）和特征性脂肪酸 3（由 iso-C(15：0) 2-OH 和/或 C(16：1)ω7c 组成；24.0%）。极性脂由双磷脂酰甘油、磷脂酰乙醇胺、一种未知氨基酸脂、一种未知氨基磷脂和三种未知极性脂组成。基于表型、系统发育和基因型数据，菌株 CNU040(T)代表 Algoriphagus 属中的一个新种，现命名为 Algoriphagus jejuensis sp. nov.。模式菌株为 CNU040(T)（ = KCTC 22647(T) = JCM 16112(T)）。
