Peng Yuxin, Cho Dong Hyun, Humaira Zalfa, Park Yu Lim, Kim Ki Hyun, Kim Cha Young, Lee Jiyoung
Korean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea.
Department of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Front Microbiol. 2024 Oct 21;15:1466733. doi: 10.3389/fmicb.2024.1466733. eCollection 2024.
, commonly known as glasswort, thrives in reclaimed land and coastal areas with high salinity, demonstrating remarkable adaptation to the arid conditions of such environments. Two aerobic, Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated TR-M5 and TR-M9, were isolated from the root of plants. These bacteria exhibit plant growth-promoting and salt tolerance-enhancing abilities, which have not been reported in other species of the genus. Both strains produce indole-3-acetic acid (IAA), a plant growth hormone, and synthesize proline, which functions as an osmoprotectant. Additionally, they possess gelatinase and cellulase activities. Cells grow in temperatures from 4 to 42°C (optimum 25°C), pH levels from 6.0 to 9.0 (optimum 7.0), and NaCl concentrations from 0 to 8.0% (optimum 6.0%). The average nucleotide identity and digital DNA-DNA hybridization values of strain TR-M5 with the most closely related type strains for which whole genomes are publicly available were 74.05-77.78% and 18.6-23.1%, respectively. Phylogenetic analysis of their 16S rRNA gene sequences revealed that strains TR-M5 and TR-M9 belong to the genus . exhibited the highest similarity, sharing a sequence identity of 98.1%. The genomes of TR-M5 and TR-M9 exhibit a G + C content of 43 mol%. This study specifically focuses on the identification and characterization of strain TR-M5 as a novel species within the genus , which we propose to name sp. nov., highlighting its potential role in enhancing plant growth and salt tolerance in saline environments. The type strain is TR-M5 (KCTC 92720 = GDMCC 1.3797).
，通常被称为海蓬子，生长在开垦地和高盐度的沿海地区，对这类环境的干旱条件表现出显著的适应性。从 植物的根部分离出两株需氧、革兰氏染色阴性、不运动、杆状的细菌菌株，分别命名为TR-M5和TR-M9。这些细菌具有促进植物生长和增强耐盐性的能力，这在该属的其他物种中尚未见报道。两株菌株都能产生植物生长激素吲哚-3-乙酸（IAA），并合成作为渗透保护剂的脯氨酸。此外，它们还具有明胶酶和纤维素酶活性。细胞在4至42°C（最适温度25°C）、pH值6.0至9.0（最适pH值7.0）和NaCl浓度0至8.0%（最适浓度6.0%）的条件下生长。菌株TR-M5与全基因组公开可用的最密切相关模式菌株的平均核苷酸同一性和数字DNA-DNA杂交值分别为74.05-77.78%和18.6-23.1%。对其16S rRNA基因序列的系统发育分析表明，菌株TR-M5和TR-M9属于 属。 表现出最高的相似性，序列同一性为98.1%。TR-M5和TR-M9的基因组G+C含量为43 mol%。本研究特别关注菌株TR-M5作为 属内一个新物种的鉴定和特征描述，我们提议将其命名为 sp. nov.，突出其在盐渍环境中增强植物生长和耐盐性的潜在作用。模式菌株为TR-M5（KCTC 92720 = GDMCC 1.3797）。
