Biotechnology Regional Innovation Center, Jeju National University, Jeju 690-756, Republic of Korea.
Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):414-419. doi: 10.1099/ijs.0.030114-0. Epub 2011 Apr 1.
A Gram-staining-negative, strictly aerobic and rod-shaped bacterium, designated strain CNURIC013(T), was isolated from seawater collected on the coast of Jeju Island, South Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CNURIC013(T) belonged to the genus Tenacibaculum, within the family Flavobacteriaceae. Sequence similarities between the novel strain and the type strains of recognized species of the genus Tenacibaculum were 93.6-96.0 %, the highest value being with Tenacibaculum litopenaei B-I(T) (96 %). The DNA G+C content of the novel strain was 34.5 mol% and the major respiratory quinone was menaquinone-6. The major fatty acids were summed feature 3 (comprising C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH; 26.0 %), iso-C(15 : 0) (24.4 %), iso-C(15 : 1) G (18.5 %) and iso-C(17 : 0) 3-OH (8.1 %). The polar lipids consisted of phosphatidylethanolamine, one unknown aminophospholipid and nine unknown polar lipids. On the basis of the phenotypic, phylogenetic and genotypic data, strain CNURIC013(T) represents a novel species within the genus Tenacibaculum, for which the name Tenacibaculum jejuense sp. nov. is proposed. The type strain is CNURIC013(T) ( = KCTC 22618(T) = JCM 15975(T)).
一株革兰氏阴性、严格需氧、杆状的细菌，被命名为 CNURIC013(T) 菌株，从韩国济州岛海岸采集的海水中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CNURIC013(T) 属于 Tenacibaculum 属，属于黄杆菌科。与新型菌株和公认的 Tenacibaculum 属的模式种的序列相似性为 93.6-96.0%，最高值是与 Tenacibaculum litopenaei B-I(T) (96%)。新型菌株的 DNA G+C 含量为 34.5 mol%，主要呼吸醌为menaquinone-6。主要脂肪酸为 sum feature 3 (包括 C(16 : 1)ω7c 和/或 iso-C(15 : 0) 2-OH；26.0%)、iso-C(15 : 0) (24.4%)、iso-C(15 : 1) G (18.5%)和 iso-C(17 : 0) 3-OH (8.1%)。极性脂类包括磷脂酰乙醇胺、一种未知的氨基磷脂和九种未知的极性脂类。基于表型、系统发育和基因型数据，菌株 CNURIC013(T) 代表 Tenacibaculum 属中的一个新种，建议命名为 Jejuense sp. nov.。该模式株为 CNURIC013(T) ( = KCTC 22618(T) = JCM 15975(T))。
