Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
Gut Liver. 2011 Mar;5(1):52-6. doi: 10.5009/gnl.2011.5.1.52. Epub 2011 Mar 16.
BACKGROUND/AIMS: Acute gastric injury by alcohol or indomethacin has been reported to be prevented by DA-9601, an extract of the herb Artemisia asiatica. Ghrelin, an endogenously produced gastrointestinal peptide hormone, has also been demonstrated to play a role in gastric mucosal defense. The aim of this study was to investigate the effects of DA-9601 on ghrelin in an acute gastric injury model induced by alcohol or indomethacin.
A total of 140 Sprague-Dawley rats were divided into two groups, a placebo group and a DA-9601-pretreated group. Thirty minutes later, half of the rats in each group received ethanol injury and the other half received indomethacin injury. Levels of serum ghrelin and gastric mucosal ghrelin mRNA were measured by ELISA and RT-PCR, respectively.
Immediately after ethanol administration, ghrelin increased in both groups pretreated with DA-9601 and placebo. However, the increase occurred more rapidly and was higher in the DA-9601-pretreated rats than in the controls that did not receive DA-9601-pretreatment. Similarly, from 30 minutes to 2 hours after indomethacin administration, the DA-9601-pretreated rats showed a significant increase in serum and gastric mucosal ghrelin concentrations, whereas placebo-pretreated rats showed only a mild increase.
DA-9601 potentiates the endogenous production and secretion of ghrelin in acute gastric injury models induced by ethanol or indomethacin.
背景/目的：已经有报道称，从蒿属植物提取的 DA-9601 可以预防酒精或消炎痛引起的急性胃损伤。内源性产生的胃肠肽激素胃饥饿素也被证明在胃黏膜防御中发挥作用。本研究旨在探讨 DA-9601 对酒精或消炎痛诱导的急性胃损伤模型中胃饥饿素的影响。
将 140 只 Sprague-Dawley 大鼠分为两组，安慰剂组和 DA-9601 预处理组。30 分钟后，每组的一半大鼠接受乙醇损伤，另一半接受消炎痛损伤。通过 ELISA 和 RT-PCR 分别测定血清胃饥饿素和胃黏膜胃饥饿素 mRNA 水平。
乙醇给药后即刻，DA-9601 预处理和安慰剂预处理的两组大鼠的胃饥饿素均增加。然而，DA-9601 预处理组的增加更快且更高。同样，从消炎痛给药后 30 分钟到 2 小时，DA-9601 预处理组的大鼠血清和胃黏膜胃饥饿素浓度显著增加，而安慰剂预处理组仅轻度增加。
DA-9601 增强了乙醇或消炎痛诱导的急性胃损伤模型中内源性胃饥饿素的产生和分泌。
