采用实验设计优化超声提取法提取中的主要化合物。
Optimization of an Ultra-Sonication Extraction Method for Major Compounds Found in Using Design of Experiment.
机构信息
Department of Chemistry, The Science Campus, College of Science Engineering and Technology, University of South Africa, Corner Christiaan de Wet and Pioneer Avenue, Florida Park, Roodepoort 1709, South Africa.
Institute for Nanotechnology and Water Sustainability, College of Science Engineering and Technology, University of South Africa, Corner Christiaan de Wet and Pioneer Avenue, Florida Park, Roodepoort 1709, South Africa.
出版信息
Molecules. 2022 Apr 29;27(9):2836. doi: 10.3390/molecules27092836.
Optimum extraction conditions are vital in quality control methods to enable accurate quantification of the compounds of interest. An ultra-sonication method was developed for the extraction of seven major compounds found in . Extraction temperature, time, power, frequency, percentage of ethanol in water and solvent to sample ratio were screened to access their significance on the percentage recovery of the compounds of interest. These parameters were screened using Descriptive screening design. Extraction temperature, solvent to sample ratio and the interaction between temperature and percentage ethanol in water were found to have a significant effect on the response. These parameters were then optimized using central composite design. The optimum conditions were found to be 66.1% ethanol in water, 70 °C temperature and 3 mL: 5 mg solvent to sample ratio. This method was successfully applied in the development of a quality control method for the seven compounds in samples.
在质量控制方法中，最佳提取条件对于准确量化感兴趣化合物至关重要。本研究开发了一种超声提取方法，用于提取 中七种主要化合物。提取温度、时间、功率、频率、水和乙醇的百分比以及溶剂与样品的比例等参数均经过筛选，以评估其对感兴趣化合物的回收率的影响。这些参数采用描述性筛选设计进行筛选。结果表明，提取温度、溶剂与样品的比例以及温度与水和乙醇百分比的相互作用对响应有显著影响。然后采用中心复合设计对这些参数进行优化。结果表明，最佳条件为 66.1%乙醇水溶液、70°C 温度和 3 mL:5 mg 溶剂与样品的比例。该方法成功应用于 样品中七种化合物的质量控制方法的开发。
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