INSERM, U1028, CNRS, UMR5292, Lyon Neuroscience Research Center, Integrated Physiology of Brain Arousal Systems, Lyon, F-69000, France.
Sleep. 2011 Apr 1;34(4):541-8. doi: 10.1093/sleep/34.4.541.
An improved animal model of obstructive sleep apnea (OSA) is needed for the development of effective pharmacotherapies. In humans, flexion of the neck and a supine position, two main pathogenic factors during human sleep, are associated with substantially greater OSA severity. We postulated that these two factors might generate OSA in animals.
We developed a restraining device for conditioning to investigate the effect of the combination of 2 body positions-prone (P) or supine (S)-and 2 head positions-with the neck flexed at right angles to the body (90°) or in extension in line with the body (180°)-during sleep in 6 cats. Polysomnography was performed twice on each cat in each of the 4 sleeping positions-P180, S180, P90, or S90. The effect of continuous positive airway pressure (CPAP) treatment was then investigated in 2 cats under the most pathogenic condition.
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Positions P180 and, S90 resulted, respectively, in the lowest and highest apnea-hypopnea index (AHI) (3 ± 1 vs 25 ± 2, P < 0.001), while P90 (18 ± 3, P<0.001) and S180 (13 ± 5, P<0.01) gave intermediate values. In position S90, an increase in slow wave sleep stage 1 (28% ± 3% vs 22% ± 3%, P<0.05) and a decrease in REM sleep (10% ± 2% vs 18% ± 2%, P<0.001) were also observed. CPAP resulted in a reduction in the AHI (8 ± 1 vs 27 ± 3, P<0.01), with the added benefit of sleep consolidation.
By mimicking human pathogenic sleep conditions, we have developed a new reversible animal model of OSA.
为了开发有效的药物治疗方法，需要建立一种改良的阻塞性睡眠呼吸暂停（OSA）动物模型。在人类中，颈部弯曲和仰卧位是人类睡眠时的两个主要致病因素，与 OSA 严重程度显著相关。我们假设这两个因素可能会导致动物发生 OSA。
我们开发了一种用于条件驯化的固定装置，以研究在睡眠期间，2 个体位（俯卧位或仰卧位）和 2 个头位（颈部与身体成直角弯曲 90°或与身体成一直线伸展 180°）与 2 个体位结合对 6 只猫的 OSA 的影响。每只猫在 4 种睡眠体位下（P180、S180、P90 或 S90）进行了两次睡眠多导生理记录仪检查。然后，在最具致病性的条件下，对 2 只猫进行持续气道正压通气（CPAP）治疗的效果进行了研究。
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体位 P180 和 S90 分别导致最低和最高的呼吸暂停低通气指数（AHI）（3 ± 1 与 25 ± 2，P<0.001），而 P90（18 ± 3，P<0.001）和 S180（13 ± 5，P<0.01）则处于中间值。在体位 S90 中，还观察到慢波睡眠第 1 期（28% ± 3%与 22% ± 3%，P<0.05）增加和 REM 睡眠（10% ± 2%与 18% ± 2%，P<0.001）减少。CPAP 导致 AHI 降低（8 ± 1 与 27 ± 3，P<0.01），同时还改善了睡眠的巩固。
通过模拟人类致病的睡眠条件，我们开发了一种新的可逆转的 OSA 动物模型。
