Department of Orthopedic Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-Cho, Shogoin, Sakyo-Ku, Kyoto 606-8507, Japan.
J Orthop Res. 2011 Oct;29(10):1572-7. doi: 10.1002/jor.21394. Epub 2011 Apr 1.
The aim of this study was to determine how alendronate (ALN) alters cartilage degeneration and periarticular bone quality in a rabbit anterior cruciate ligament transection (ACLT) model of osteoarthritis (OA). Thirty rabbits underwent an ACLT on the left knee and a sham operation on the right knee. Fifteen rabbits received weekly subcutaneous injections of ALN (0.14 mg/kg) and 15 rabbits (the control [cont] group) received saline. Animal knees were divided into four groups: cont/sham, cont/ACLT, ALN/sham, and ALN/ACLT. Histological, radiological, and immunohistochemical indices were evaluated for each group. Bone volume ratios by micro-computed tomography showed that ALN prevented periarticular bone loss. Histologically, the cont/ACLT group had significantly worse cartilage damage than the cont/sham group 12 weeks after the surgery. However, the ALN/ACLT group had mild cartilage degeneration compared with that of the ALN/sham group. Immunohistochemical analysis showed that ALN suppressed the expression of matrix metalloproteinase-13, interleukin-1β, type-X collagen, vascular endothelial growth factor, and receptor activator of nuclear factor κB ligand in OA cartilage. ALN had a chondroprotective effect in an experimental rabbit model of OA.
本研究旨在探讨阿仑膦酸钠（ALN）在兔前交叉韧带切断（ACLT）骨关节炎（OA）模型中对软骨退变和关节周围骨质量的影响。30 只兔子的左膝关节行 ACLT，右膝关节行假手术。15 只兔子每周接受皮下注射 ALN（0.14mg/kg），15 只兔子（对照组）接受生理盐水。将动物膝关节分为四组：对照组/假手术组、对照组/ACLT 组、ALN/假手术组和 ALN/ACLT 组。对每组进行组织学、影像学和免疫组织化学评估。骨体积比的微计算机断层扫描显示，ALN 可预防关节周围骨丢失。组织学检查显示，手术后 12 周，ACLT 组的软骨损伤明显比假手术组严重。然而，与 ALN/假手术组相比，ALN/ACLT 组的软骨退变较轻。免疫组织化学分析显示，ALN 抑制了 OA 软骨中基质金属蛋白酶-13、白细胞介素-1β、X 型胶原、血管内皮生长因子和核因子κB 配体受体激活蛋白的表达。ALN 对兔 OA 实验模型具有软骨保护作用。
