Ribeiro T P, Sousa T R, Arruda A S, Peixoto N, Gonçalves P J, Almeida L M
Laboratório de Biologia Molecular de Plantas, Universidade Estadual de Goiás, Ipameri, GO, Brazil.
Laboratório de Fotofísica, Instituto de Física, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Braz J Biol. 2016 Feb;76(1):245-9. doi: 10.1590/1519-6984.20114. Epub 2016 Jan 22.
The latex obtained from Hancornia speciosa Gomes (Mangabeira tree) is widely used in traditional medicine to treat a variety of diseases, including diarrhea, ulcer, gastritis, tuberculosis, acne and warts. In this study, the cytotoxicity and genotoxicity effects of H. speciosa latex on the root meristem cells of Allium cepa were examined. Onion bulbs were exposed to different concentrations of latex and then submitted to microscopic analysis using Giemsa stain. Water was used as a negative control and sodium azide as a positive control. The results showed that, under the testing conditions, the mitotic index (MI) of the onion roots submitted to latex treatment did not differ significantly from the negative control, which suggests that the latex is not cytotoxic. Low incidence of chromosome aberrations in the cells treated with H. speciosa latex was also observed, indicating that the latex does not have genotoxic effect either. The MI and the chromosome aberration frequency responded to the latex concentration, requiring more studies to evaluate the dosage effect on genotoxicity. The results indicate that in tested concentrations H. speciosa latex is probably not harmful to human health and may be potentially used in medicine.
从曼加贝拉树（Hancornia speciosa Gomes）中提取的乳胶在传统医学中被广泛用于治疗多种疾病，包括腹泻、溃疡、胃炎、肺结核、痤疮和疣。在本研究中，检测了曼加贝拉树乳胶对洋葱根尖分生组织细胞的细胞毒性和遗传毒性作用。将洋葱鳞茎暴露于不同浓度的乳胶中，然后使用吉姆萨染色进行显微镜分析。以水作为阴性对照，叠氮化钠作为阳性对照。结果表明，在测试条件下，接受乳胶处理的洋葱根的有丝分裂指数（MI）与阴性对照无显著差异，这表明乳胶没有细胞毒性。在用曼加贝拉树乳胶处理的细胞中也观察到染色体畸变的发生率较低，表明乳胶也没有遗传毒性。MI和染色体畸变频率对乳胶浓度有反应，需要更多研究来评估其对遗传毒性的剂量效应。结果表明，在测试浓度下，曼加贝拉树乳胶可能对人体健康无害，并且可能有潜在的医学用途。
