Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164-6120, USA.
Bioresour Technol. 2011 May;102(10):6134-40. doi: 10.1016/j.biortech.2011.02.081. Epub 2011 Apr 3.
This paper explores the use of the hydrolysate from the dilute sulfuric acid pretreatment of wheat straw for microbial oil production. The resulting hydrolysate was composed of pentoses (24.3g/L) and hexoses (4.9 g/L), along with some other degradation products, such as acetic acid, furfural, and hydroxymethylfurfural (HMF). Five oleaginous yeast strains, Cryptococcus curvatus, Rhodotorula glutinis, Rhodosporidium toruloides, Lipomyces starkeyi, and Yarrowia lipolytica, were evaluated by using this hydrolysate as substrates. The results showed that all of these strains could use the detoxified hydrolysate to produce lipids while except R. toruloides non-detoxified hydrolysate could also be used for the growth of all of the selective yeast strains. C. curvatus showed the highest lipid concentrations in medium on both the detoxified (4.2g/L) and non-detoxified (5.8 g/L) hydrolysates. And the inhibitory effect studies on C. curvatus indicated HMF had insignificant impacts at a concentration of up to 3g/L while furfural inhibited cell growth and lipid content by 72.0% and 62.0% at 1g/L, respectively. Our work demonstrates that lipid production is a promising alternative to utilize hemicellulosic sugars obtained during pretreatment of lignocellulosic materials.
本文探讨了利用小麦秸秆稀硫酸预处理水解产物进行微生物油脂生产的方法。所得水解产物由戊糖（24.3g/L）和己糖（4.9g/L）以及一些其他降解产物组成，如乙酸、糠醛和羟甲基糠醛（HMF）。使用该水解物作为底物，评估了五种产油酵母菌株，包括皱褶假丝酵母（Cryptococcus curvatus）、粘红酵母（Rhodotorula glutinis）、罗伦隐球酵母（Rhodosporidium toruloides）、汉逊德巴利酵母（Lipomyces starkeyi）和产脂耶氏酵母（Yarrowia lipolytica）。结果表明，除罗伦隐球酵母外，所有这些菌株都可以利用解毒水解物来生产油脂，而非解毒水解物也可以用于所有选择性酵母菌株的生长。在解毒（4.2g/L）和非解毒（5.8g/L）水解物中，皱褶假丝酵母的细胞浓度最高（4.2g/L）。对皱褶假丝酵母的抑制作用研究表明，在 3g/L 浓度下，HMF 几乎没有影响，而糠醛在 1g/L 时分别抑制细胞生长和脂质含量 72.0%和 62.0%。我们的工作表明，利用木质纤维素材料预处理过程中获得的半纤维素糖进行脂质生产是一种很有前途的替代方法。
