Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.
Department of Biotechnology, Korea University, Seoul 02841, Korea.
Int J Mol Sci. 2022 Apr 26;23(9):4761. doi: 10.3390/ijms23094761.
, the non-conventional yeast capable of high lipogenesis, is a microbial chassis for producing lipid-based biofuels and chemicals from renewable resources such as lignocellulosic biomass. However, the low tolerance of against furfural, a major inhibitory furan aldehyde derived from the pretreatment processes of lignocellulosic biomass, has restricted the efficient conversion of lignocellulosic hydrolysates. In this study, the furfural tolerance of has been improved by supporting its endogenous detoxification mechanism. Specifically, the endogenous genes encoding the aldehyde dehydrogenase family proteins were overexpressed in to support the conversion of furfural to furoic acid. Among them, YALI0E15400p (FALDH2) has shown the highest conversion rate of furfural to furoic acid and resulted in two-fold increased cell growth and lipid production in the presence of 0.4 g/L of furfural. To our knowledge, this is the first report to identify the native furfural detoxification mechanism and increase furfural resistance through rational engineering in . Overall, these results will improve the potential of to produce lipids and other value-added chemicals from a carbon-neutral feedstock of lignocellulosic biomass.
产脂非传统酵母能够进行高效的脂类生物合成，是利用可再生资源（如木质纤维素生物质）生产基于脂类的生物燃料和化学品的微生物底盘。然而，木质纤维素生物质预处理过程中产生的主要抑制呋喃醛——糠醛，对 的低容忍度限制了木质纤维素水解物的有效转化。在本研究中，通过支持其内源解毒机制来提高 对糠醛的耐受性。具体来说，过量表达编码醛脱氢酶家族蛋白的内源基因，以支持糠醛转化为糠酸。其中，YALI0E15400p（FALDH2）表现出最高的糠醛转化为糠酸的转化率，并且在 0.4 g/L 糠醛存在下，细胞生长和脂类产量增加了两倍。据我们所知，这是首次在 中通过合理的工程设计来鉴定天然糠醛解毒机制并提高糠醛抗性的报道。总的来说，这些结果将提高 利用木质纤维素生物质这一碳中和原料生产脂类和其他高附加值化学品的潜力。
